Protective Effect of Yinhua Miyanling Tablet on Lipopolysaccharide-Induced Inflammation through Suppression of NLRP3/Caspase-1 Inflammasome in Human Peripheral Blood Mononuclear Cells

Figure 5

Effect of YMT on the protein expression of NLRP3, Caspase-1, and NLRP1 in PBMC. Cells were pretreated with 1000 ng/mL LPS for 1 hour prior to treatment with different concentration of YMT (0.19, 0.38, and 0.75 mg/mL) for 18 hours. The last group of cells was stimulated with 1000 ng/mL LPS for 1 hour. The proteins were extracted from the cell lysates after treatment. Western blot analyses were performed using NLRP3, Caspase-1, and NLRP1 antibodies. β-actin served as the housekeeping control.