Evaluation of Medicinal Categorization of Atractylodes japonica Koidz. by Using Internal Transcribed Spacer Sequencing Analysis and HPLC Fingerprinting Combined with Statistical Tools

Table 4

Genotypes of ITS nucleotide sequences deposited in the NCBI GenBank for recognition in the Atractylodes species.

Nucleotide position

69

71

81

104

117

120

121

128

134

135

141

142

143

145

149

157

170

177

222

226

259

260

279

465

481

494

502

529

530

534

544

560

572

599

658

659

666

Genotype

Type 1

T

A

G

C

G

Y

C

G

M

Y

R

T

C

Y

Y

T

T

R

A

A

C

G

T

W

Y

Y

R

Y

R

Y

A

Y

W

C

C

G

C

Type 2

C

A

A

C

G

C

A

G

C

C

G

C

A

C

C

C

C

G

A

G

C

R

T

G

C

C

G

C

G

C

C

C

T

T

T

G

Y

Type 3

T

G

G

C

G

C

C

G

C

C

G

T

C

C

C

T

T

A

A

A

G

G

T

A

C

C

G

T

G

C

A

C

A

C

C

G

C

Type 4

T

A

G

T

A

C

C

A

C

C

G

T

C

C

T

T

T

G

C

A

C

G

C

T

C

C

G

C

G

C

A

C

T

C

C

A

C

Type 5

T

A

G

T

A

C

C

A

C

C

G

T

C

C

T

T

T

G

C

A

C

G

C

T

C

C

G

C

G

C

A

C

T

C

C

A

C

Bold characters indicate nucleotide additives, M = A and C; R = A and G; W = A and T; Y = C and T. Type 1 genotype resulted from deposited ITS nucleotide sequences in NCBI Genbank of Atractylodes japonica. Type 2 and type 3 represent A. macrocephala and A. lancea. Type 4 and type 5 represent A. chinensis and A. koreana.