Ultrastructure of a Mobile Threadlike Tissue Floating in a Lymph Vessel

Figure 2

Images of a harvested lymph duct in which a PV was stained with Alcian blue. (a) A piece of the lymph vessel (LV) was harvested and image was taken with a phase contrast microscope. The PV is clearly seen due to the Alcian blue staining. (b) The stained PV was extracted from the lymph vessel with a forceps. It was treated with DAPI to stain its nuclei. (c) The boxed region of the PV in (b) was examined with a confocal microscope. The rod-shaped nuclei of the endothelial cells of the PV were observed at the 15 μm depth from the surface of the stained threadlike structure whose thickness was 23 μm. The PV became thicker because it was covered with lymphocytes.

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