Pinoresinol Diglucoside Alleviates oxLDL-Induced Dysfunction in Human Umbilical Vein Endothelial Cells

Figure 2

Pinoresinol diglucoside alleviates oxidative stress caused by oxLDL. (a) Representative pictures of HUVECs stained with DCFH-DA after being cultured without treatment, with 100 μg/mL of oxLDL, 1 μmol/L of PD, and 100 μg/mL of oxLDL plus 1 μmol/L of PD for 24 hrs. (b) The relative ROS levels of HUVECs were analyzed by flow cytometry following DCFH-DA staining after being cultured without treatment, with 40 μg/mL of oxLDL, 1 μmol/L of PD, and 40 μg/mL of oxLDL plus 1 μmol/L of PD for 24 hrs. (c) The lipid peroxidation was assessed by measuring the contents of malondialdehyde in HUVECs treated with oxLDL and/or PD. Experiments were performed 3 independent times with 3 replications. compared to control cells; compared to oxLDL-treated cells.

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