Synergistic Effect of Ferulic Acid and Z-Ligustilide, Major Components of A. sinensis, on Regulating Cold-Sensing Protein TRPM8 and TPRA1 In Vitro

Figure 4

Inhibition effects of ferulic acid, Z-ligustilide, or their combination on GTP-RhoA level and phosphorylation of MLC₂₀ with absence or presence of WS-12 or ASP 7663. HASMC were stimulated by ferulic acid or Z-ligustilide alone (a), 50 μM WS-12 (b), or 50 μM ASP 7663 (c) for 10 minutes in the absence or presence of ferulic acid, Z-ligustilide, or their combination. Data are expressed in % of GTP-RhoA expression normalized to total RhoA expression or phosphorylation of MLC₂₀ normalized to total MLC₂₀ expression. Immunofluorescence images of phosphorylation of MLC₂₀ were also captured. Bars are mean values from 3 individual samples.Significant difference compared to control ().Significant difference compared to ferulic acid group or Z-ligustilide group ().

(a)

(b)

(c)