Formula Compatibility Identification of Dachengqi Decoction Based on the Effects of Absorbed Components in Cerulein-Injured Pancreatic AR42J Cells

Figure 2

The synergistic effects of rhein, honokiol, and naringin from DCQD on the apoptosis-necrosis cellular switch in cerulein-induced AR42J cells. Cells were pretreated with or without the three major components with the peak concentrations individually or in combination for 30 min and then coincubated with 10 nM cerulein for 24 h. Annexin V/PI staining was performed and flow cytometry analyses were used. Each panel is divided into four regions: viable cells (annexin V⁻/propidium iodide (PI)⁻) are located in the lower left quadrant, early apoptotic cells (annexin V⁺/PI⁻) in the lower right quadrant, late apoptotic and necrotic cells (annexin V⁺/PI⁺) in the upper right quadrant, and primary necrotic cells (annexin V⁻/PI⁺) in the upper left quadrant.

(a) Rhein

(b) Honokiol

(c) Naringin

(d) Rhein + honokiol

(e) Rhein + naringin

(f) Honokiol + naringin

(g) Rhein + honokiol + naringin

(h) Ten compounds combinations

(i) DCQD

(j) Cerulein

(k) Normal