Formula Compatibility Identification of Dachengqi Decoction Based on the Effects of Absorbed Components in Cerulein-Injured Pancreatic AR42J Cells

Table 2

The effects of rhein, honokiol, and naringin individually or in combination on cerulein-induced AR42J cell death and LDH release.

Component	Cell viability (%)	LDH release (%)
R	72.96 ± 1.38	22.57 ± 0.79
H	71.02 ± 0.79	24.63 ± 0.59
N	70.40 ± 1.50	26.83 ± 1.38
R plus H	74.43 ± 2.13	21.09 ± 2.03
R plus N	72.46 ± 1.34	22.04 ± 0.46
N plus H	72.15 ± 0.67	24.39 ± 0.73
R plus H plus N	76.02 ± 0.93	20.73 ± 1.37
Ten components	78.95 ± 1.88	17.07 ± 0.87
DCQD	80.44 ± 2.38	15.85 ± 0.45
Cerulein alone	63.47 ± 1.03	40.24 ± 2.03
Normal	92.30 ± 1.32	5.31 ± 0.23

R = rhein, H = honokiol, N = naringin, DCQD = Dachengqi decoction, and LDH = lactate dehydrogenase. Cells were pretreated with the components with the peak concentrations for 30 min and then coincubated with 10 nM cerulein for 24 h. After cerulein is added, cell viability is examined by WST-8 assay. Necrotic cell death was assessed by the release of LDH from the cytosol of damaged cells into the supernatant using the LDH Cytotoxicity Detection Kit. The results are mean ± SD. versus cerulein alone-treated group, versus rhein-treated group, and versus DCQD-treated group.