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Evidence-Based Complementary and Alternative Medicine
Volume 2016, Article ID 3251046, 13 pages
http://dx.doi.org/10.1155/2016/3251046
Research Article

Chinese Herbal Mixture, Tien-Hsien Liquid, Induces G2/M Cycle Arrest and Radiosensitivity in MCF-7 Human Breast Cancer Cells through Mechanisms Involving DNMT1 and Rad51 Downregulation

1Comprehensive Cancer Center, Taipei Medical University, Taipei 11031, Taiwan
2Cancer Center, Wan Fang Hospital, Taipei Medical University, Taipei 11696, Taiwan
3Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
4Division of Hematology and Medical Oncology, Department of Internal Medicine, Wan Fang Hospital, Taipei Medical University, Taipei 11696, Taiwan
5Formosa Cancer Foundation, Taipei 10597, Taiwan
6The Ph.D. Program for Translational Medicine, Taipei Medical University-Academia Sinica, Taipei 11031, Taiwan
7Division of Radiation Oncology, Department of Oncology, Taipei Veterans General Hospital, Taipei 11217, Taiwan
8National Institute of Cancer Research, National Health Research Institutes, Miaoli County 35053, Taiwan

Received 24 January 2016; Revised 23 May 2016; Accepted 22 June 2016

Academic Editor: Ghee T. Tan

Copyright © 2016 Chih-Jung Yao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. W.-H. Kuo, C.-A. Yao, C. H. Lin, and K.-J. Chang, “Safety and efficacy of Tien-Hsien Liquid Practical in patients with refractory metastatic breast cancer: a randomized, double-blind, placebo-controlled, parallel-group, phase IIa trial,” Evidence-Based Complementary and Alternative Medicine, vol. 2012, Article ID 803239, 8 pages, 2012. View at Publisher · View at Google Scholar · View at Scopus
  2. A. Sun, J.-S. Chia, W.-B. Wang, and C.-P. Chiang, “Immunomodulating effects of ‘Tien-Hsien liquid’ on peripheral blood mononuclear cells and T-lymphocytes from patients with recurrent aphthous ulcerations,” American Journal of Chinese Medicine, vol. 32, no. 2, pp. 221–234, 2004. View at Publisher · View at Google Scholar · View at Scopus
  3. A. Sun, J.-S. Chia, C.-P. Chiang et al., “The Chinese herbal medicine Tien-Hsien liquid inhibits cell growth and induces apoptosis in a wide variety of human cancer cells,” Journal of Alternative and Complementary Medicine, vol. 11, no. 2, pp. 245–256, 2005. View at Publisher · View at Google Scholar · View at Scopus
  4. J.-S. Chia, J.-L. Du, W.-B. Hsu, A. Sun, C.-P. Chiang, and W.-B. Wang, “Inhibition of metastasis, angiogenesis, and tumor growth by Chinese herbal cocktail Tien-Hsien Liquid,” BMC Cancer, vol. 10, article 175, 2010. View at Publisher · View at Google Scholar · View at Scopus
  5. T.-Y. Lai, C.-J. Yao, C.-M. Yang et al., “Targeting PML-RARα and oncogenic signaling pathways by Chinese herbal mixture Tien-Hsien liquid in acute promyelocytic leukemia NB4 cells,” Evidence-Based Complementary and Alternative Medicine, vol. 2011, Article ID 984154, 11 pages, 2011. View at Publisher · View at Google Scholar · View at Scopus
  6. D. Subramaniam, R. Thombre, A. Dhar, and S. Anant, “DNA methyltransferases: a novel target for prevention and therapy,” Frontiers in Oncology, vol. 4, article 80, 2014. View at Publisher · View at Google Scholar · View at Scopus
  7. A. T. Agoston, P. Argani, S. Yegnasubramanian et al., “Increased protein stability causes DNA methyltransferase 1 dysregulation in breast cancer,” The Journal of Biological Chemistry, vol. 280, no. 18, pp. 18302–18310, 2005. View at Publisher · View at Google Scholar · View at Scopus
  8. S. P. Zielske, “Epigenetic dna methylation in radiation biology: on the field or on the sidelines?” Journal of Cellular Biochemistry, vol. 116, no. 2, pp. 212–217, 2015. View at Publisher · View at Google Scholar · View at Scopus
  9. V. Singh, P. Sharma, and N. Capalash, “DNA methyltransferase-1 inhibitors as epigenetic therapy for cancer,” Current Cancer Drug Targets, vol. 13, no. 4, pp. 379–399, 2013. View at Publisher · View at Google Scholar · View at Scopus
  10. C. Pellerito, O. Morana, F. Ferrante et al., “Synthesis, chemical characterization, computational studies and biological activity of new DNA methyltransferases (DNMTs) specific inhibitor. Epigenetic regulation as a new and potential approach to cancer therapy,” Journal of Inorganic Biochemistry, vol. 150, article 9731, pp. 18–27, 2015. View at Publisher · View at Google Scholar · View at Scopus
  11. Z. Yu, Q. Xiao, L. Zhao et al., “DNA methyltransferase 1/3a overexpression in sporadic breast cancer is associated with reduced expression of estrogen receptor-alpha/breast cancer susceptibility gene 1 and poor prognosis,” Molecular Carcinogenesis, vol. 54, no. 9, pp. 707–719, 2015. View at Publisher · View at Google Scholar · View at Scopus
  12. S. Vijayaraghavalu, J. K. Dermawan, V. Cheriyath, and V. Labhasetwar, “Highly synergistic effect of sequential treatment with epigenetic and anticancer drugs to overcome drug resistance in breast cancer cells is mediated via activation of p21 gene expression leading to G2/M cycle arrest,” Molecular Pharmaceutics, vol. 10, no. 1, pp. 337–352, 2013. View at Publisher · View at Google Scholar · View at Scopus
  13. H. J. Kim, J. H. Kim, E. K. Chie, P. Da Young, I. A. Kim, and I. H. Kim, “DNMT (DNA methyltransferase) inhibitors radiosensitize human cancer cells by suppressing DNA repair activity,” Radiation Oncology, vol. 7, article 39, 2012. View at Publisher · View at Google Scholar · View at Scopus
  14. H. Qiu, M. Yashiro, O. Shinto, T. Matsuzaki, and K. Hirakawa, “DNA methyltransferase inhibitor 5-aza-CdR enhances the radiosensitivity of gastric cancer cells,” Cancer Science, vol. 100, no. 1, pp. 181–188, 2009. View at Publisher · View at Google Scholar · View at Scopus
  15. A. B. Leigh, H. P. Cheung, L. Z. Lin et al., “Comprehensive and holistic analysis of HT-29 colorectal cancer cells and tumor-bearing nude mouse model: interactions among fractions derived from the Chinese medicine formula Tian Xian liquid in effects on human colorectal carcinoma,” Integrative Cancer Therapies, In press.
  16. P. Skehan, R. Storeng, D. Scudiero et al., “New colorimetric cytotoxicity assay for anticancer-drug screening,” Journal of the National Cancer Institute, vol. 82, no. 13, pp. 1107–1112, 1990. View at Publisher · View at Google Scholar · View at Scopus
  17. V. Vichai and K. Kirtikara, “Sulforhodamine B colorimetric assay for cytotoxicity screening,” Nature Protocols, vol. 1, no. 3, pp. 1112–1116, 2006. View at Publisher · View at Google Scholar · View at Scopus
  18. W.-T. Hsieh, K.-Y. Huang, H.-Y. Lin, and J.-G. Chung, “Physalis angulata induced G2/M phase arrest in human breast cancer cells,” Food and Chemical Toxicology, vol. 44, no. 7, pp. 974–983, 2006. View at Publisher · View at Google Scholar · View at Scopus
  19. D. L. Clemens, L. E. Calisto, M. F. Sorrell, and D. J. Tuma, “Ethanol metabolism results in a G2/M cell-cycle arrest in recombinant Hep G2 cells,” Hepatology, vol. 38, no. 2, pp. 385–393, 2003. View at Publisher · View at Google Scholar · View at Scopus
  20. V. A. J. Smits, R. Klompmaker, T. Vallenius, G. Rijksen, T. P. Mäkelä, and R. H. Medema, “p21 Inhibits Thr161 phosphorylation of Cdc2 to enforce the G2 DNA damage checkpoint,” The Journal of Biological Chemistry, vol. 275, no. 39, pp. 30638–30643, 2000. View at Publisher · View at Google Scholar · View at Scopus
  21. J. Yu, Y. Peng, L.-C. Wu et al., “Curcumin down-regulates DNA methyltransferase 1 and plays an anti-leukemic role in acute myeloid leukemia,” PLoS ONE, vol. 8, no. 2, Article ID e55934, 2013. View at Publisher · View at Google Scholar · View at Scopus
  22. E. S. Knudsen and K. E. Knudsen, “Tailoring to RB: tumour suppressor status and therapeutic response,” Nature Reviews Cancer, vol. 8, no. 9, pp. 714–724, 2008. View at Publisher · View at Google Scholar · View at Scopus
  23. K. S. Saini, S. Loi, E. de Azambuja et al., “Targeting the PI3K/AKT/mTOR and Raf/MEK/ERK pathways in the treatment of breast cancer,” Cancer Treatment Reviews, vol. 39, no. 8, pp. 935–946, 2013. View at Publisher · View at Google Scholar · View at Scopus
  24. H. Choy, F. F. Rodriguez, S. Koester, S. Hilsenbeck, and D. D. Von Hoff, “Investigation of taxol as a potential radiation sensitizer,” Cancer, vol. 71, no. 11, pp. 3774–3778, 1993. View at Google Scholar · View at Scopus
  25. D. Thummuri, S. Kumar, S. K. Surapaneni, and K. Tikoo, “Epigenetic regulation of protein tyrosine phosphatase PTPN12 in triple-negative breast cancer,” Life Sciences, vol. 130, pp. 73–80, 2015. View at Publisher · View at Google Scholar · View at Scopus
  26. M. Billam, M. D. Sobolewski, and N. E. Davidson, “Effects of a novel DNA methyltransferase inhibitor zebularine on human breast cancer cells,” Breast Cancer Research and Treatment, vol. 120, no. 3, pp. 581–592, 2010. View at Publisher · View at Google Scholar · View at Scopus
  27. M. Menschikowski, A. Hagelgans, B. Nacke, C. Jandeck, O. Sukocheva, and G. Siegert, “Epigenetic control of phospholipase A2 receptor expression in mammary cancer cells,” BMC Cancer, vol. 15, article 971, 2015. View at Publisher · View at Google Scholar · View at Scopus
  28. M. T. McCabe, J. N. Davis, and M. L. Day, “Regulation of DNA methyltransferase 1 by the pRb/E2F1 pathway,” Cancer Research, vol. 65, no. 9, pp. 3624–3632, 2005. View at Publisher · View at Google Scholar · View at Scopus
  29. A. T. Agoston, P. Argani, A. M. De Marzo, J. L. Hicks, and W. G. Nelson, “Retinoblastoma pathway dysregulation causes DNA methyltransferase 1 overexpression in cancer via MAD2-mediated inhibition of the anaphase-promoting complex,” The American Journal of Pathology, vol. 170, no. 5, pp. 1585–1593, 2007. View at Publisher · View at Google Scholar · View at Scopus
  30. Z. Mao, Y. Jiang, X. Liu, A. Seluanov, and V. Gorbunova, “DNA repair by homologous recombination, but not by nonhomologous end joining, is elevated in breast cancer cells,” Neoplasia, vol. 11, no. 7, pp. 683–691, 2009. View at Publisher · View at Google Scholar · View at Scopus
  31. J. Wang, Q. Liu, and Q. Yang, “Radiosensitization effects of berberine on human breast cancer cells,” International Journal of Molecular Medicine, vol. 30, no. 5, pp. 1166–1172, 2012. View at Publisher · View at Google Scholar · View at Scopus
  32. C. M. Hine, A. Seluanov, and V. Gorbunova, “Rad51 promoter-targeted gene therapy is effective for in vivo visualization and treatment of cancer,” Molecular Therapy, vol. 20, no. 2, pp. 347–355, 2012. View at Publisher · View at Google Scholar · View at Scopus
  33. A. Nogueira, R. Catarino, A. Coelho, A. Araújo, M. Gomes, and R. Medeiros, “Influence of DNA repair RAD51 gene variants in overall survival of non-small cell lung cancer patients treated with first line chemotherapy,” Cancer Chemotherapy and Pharmacology, vol. 66, no. 3, pp. 501–506, 2010. View at Publisher · View at Google Scholar · View at Scopus
  34. H. Maacke, S. Opitz, K. Jost et al., “Over-expression of wild-type Rad51 correlates with histological grading of invasive ductal breast cancer,” International Journal of Cancer, vol. 88, no. 6, pp. 907–913, 2000. View at Publisher · View at Google Scholar · View at Scopus
  35. L.-Q. Du, Y. Wang, H. Wang, J. Cao, Q. Liu, and F.-Y. Fan, “Knockdown of Rad51 expression induces radiation- and chemo-sensitivity in osteosarcoma cells,” Medical Oncology, vol. 28, no. 4, pp. 1481–1487, 2011. View at Publisher · View at Google Scholar · View at Scopus
  36. K. R. Bauer, M. Brown, R. D. Cress, C. A. Parise, and V. Caggiano, “Descriptive analysis of estrogen receptor (ER)-negative, progesterone receptor (PR)-negative, and HER2-negative invasive breast cancer, the so-called triple-negative phenotype: a population-based study from the California Cancer Registry,” Cancer, vol. 109, no. 9, pp. 1721–1728, 2007. View at Publisher · View at Google Scholar · View at Scopus
  37. D. S. Choi, E. Blanco, Y.-S. Kim et al., “Chloroquine eliminates cancer stem cells through deregulation of Jak2 and DNMT1,” Stem Cells, vol. 32, no. 9, pp. 2309–2323, 2014. View at Publisher · View at Google Scholar · View at Scopus
  38. H. Tang, P. Liu, L. Yang et al., “miR-185 suppresses tumor proliferation by directly targeting E2F6 and DNMT1 and indirectly upregulating BRCA1 in triple-negative breast cancer,” Molecular Cancer Therapeutics, vol. 13, no. 12, pp. 3185–3197, 2014. View at Publisher · View at Google Scholar · View at Scopus
  39. R. Pathania, S. Ramachandran, S. Elangovan et al., “DNMT1 is essential for mammary and cancer stem cell maintenance and tumorigenesis,” Nature Communications, vol. 6, article 6910, 2015. View at Publisher · View at Google Scholar · View at Scopus
  40. C.-J. Yao, C.-T. Yeh, L.-M. Lee et al., “Elimination of cancer stem-like side population cells in hepatoma cell lines by Chinese herbal mixture Tien-Hsien Liquid,” Evidence-Based Complementary and Alternative Medicine, vol. 2012, Article ID 617085, 10 pages, 2012. View at Publisher · View at Google Scholar · View at Scopus
  41. S.-Y. Li, R. Sun, H.-X. Wang et al., “Combination therapy with epigenetic-targeted and chemotherapeutic drugs delivered by nanoparticles to enhance the chemotherapy response and overcome resistance by breast cancer stem cells,” Journal of Controlled Release, vol. 205, pp. 7–14, 2015. View at Publisher · View at Google Scholar · View at Scopus