Evaluation of the In Vivo Therapeutic Effects of Radix Paeoniae Rubra Ethanol Extract with the Hypoglycemic Activities Measured from Multiple Cell-Based Assays

Figure 2

Multiple hypoglycemic activities of PRExt. (a) A dose-dependent suppressive effect of PRExt on PEPCK mRNA expression. Insulin (100 nM) served as a reference drug. Data are presented as the mean ± SEM (/group). when compared with mRNA levels in H4IIE cells treated with dexamethasone and 8-bromo-cAMP. (b) The glucose uptake activity of L6 myotubes exposed to PRExt or insulin (100 nM) for 30 minutes prior to performing a 2-NBDG (100 μM) uptake assay. Data are presented as the mean ± SEM (/group). and when compared with untreated L6 myotubes. (c) The insulinotropic effect of PRExt on BRIN-BD11 cells under hyperglycemic condition (16.7 mM glucose). Exendin-4 (100 nM) was used as a reference drug. Data are presented as the mean ± SEM (/group). , , and when compared with insulin levels under hyperglycemic conditions (16.7 mM glucose).

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