DNA Protection against Oxidative Damage Using the Hydroalcoholic Extract of Garcinia mangostana and Alpha-Mangostin
Table 1
Induction of his+ revertants in S. typhimurium strains by Garcinia mangostana extract with and without metabolic activation (S9 mix).
S. typhimurium strains
Substance
Concentration (µg/plate)
TA100
TA98
Rev./plate
MI
Rev./plate
MI
Without metabolic activation (−S9)
NC extract
—
95.3 ± 19.9
—
30.0 ± 5.2
—
4
90.3 ± 10.1
0.95
28.0 ± 2.7
0.93
8
102.0 ± 16.6
1.07
20.3 ± 4.6
0.68
12
99.3 ± 5.03
1.04
29.0 ± 8.7
0.97
16
95.0 ± 19.5
1.00
28.3 ± 6.8
0.94
20
81.3 ± 57.1
0.85
19.7 ± 9.0
0.66
PC
0.5 (4NQO)/1 (NaN3)
479.7 ± 57.1
5.03
362.7 ± 34.5
12.09
With metabolic activation (+S9)
NC extract
—
90.3 ± 30.4
—
49.3 ± 9.2
—
100
81.0 ± 12.3
0.90
46.3 ± 7.5
0.94
200
82.0 ± 9.5
0.91
47.0 ± 4.6
1.14
300
86.7 ± 15.7
0.96
51.3 ± 4.0
0.95
400
80.3 ± 13.5
0.89
58.0 ± 13.9
0.92
500
79.0 ± 8.7
0.88
66.0 ± 24.3
1.14
PC
1 (AFB1)
296.3 ± 62.7
3.28
569.7 ± 15.7
11.55
Number of revertants/plate: mean of three independent experiments ± SD; MI: mutagenic index (number of his+ induced in the sample/number of spontaneous his+ in the negative control); NC: negative control DMSO (10 µL) used as solvent for the extract; PC: positive control (−S9) sodium azide to TA100; 4-NQO to TA98; (+S9) aflatoxin B1. Data significantly different in relation to the negative control ; .
