Evidence-Based Complementary and Alternative Medicine / 2016 / Article / Fig 4

Research Article

Anti-Inflammatory Activity of Bee Venom in BV2 Microglial Cells: Mediation of MyD88-Dependent NF-κB Signaling Pathway

Figure 4

Bee venom inhibits the expression level of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) mRNA in LPS-stimulated BV2 microglia. Dose-dependent inhibition of TNF-α (a) and IL-6 (b) mRNA expression were assessed using quantitative real-time PCR. BV2 cells were pretreated with bee venom for 30 min, and then 0.1 μg/mL LPS was added and the cells were incubated for an additional 24 h. The total RNA preparation and real-time PCR were performed as described in Section 2. GAPDH was used as an internal control, and relative expression levels of TNF-α and IL-6 mRNA were calculated by normalization to GAPDH. The data are presented as mean ± SEM of three to five experiments. versus LPS alone.

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