Evidence-Based Complementary and Alternative Medicine / 2016 / Article / Fig 5

Research Article

Anti-Inflammatory Activity of Bee Venom in BV2 Microglial Cells: Mediation of MyD88-Dependent NF-κB Signaling Pathway

Figure 5

Inhibition of degradation of IκB-α, phosphorylation of IKKα/β, nuclear translocation of the p65 subunit of NF-κB/Rel, and NF-κB transcriptional activity by bee venom in LPS-stimulated BV2 microglia. The inhibitory effect of bee venom on the nuclear translocation of the p65 subunit of NF-κB/Rel, degradation of IκB-α (a), phosphorylation of IKKα/β (b), and NF-κB transcriptional activity (c). BV2 cells were pretreated with bee venom or vehicle for 30 min and then stimulated with 0.1 μg/mL LPS for the indicated times. The protein extraction and SDS-PAGE methods are described in Section 2. The β-actin was used as an internal loading control for the immunoblot analysis. The effect of bee venom on NF-κB transcriptional activity was determined by firefly luciferase activity by using a luminometer. and versus LPS alone.
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