Anti-Inflammatory Activity of Bee Venom in BV2 Microglial Cells: Mediation of MyD88-Dependent NF-κB Signaling Pathway

Figure 6

Bee venom modulates MAPK and TAK1 phosphorylation in LPS-stimulated BV2 microglia. The inhibitory effects of bee venom on the phosphorylation of extracellular-signal regulated kinase 1/2 (ERK1/2), c-jun N-terminal kinase (JNK), p38 mitogen-activated protein kinase (p38-MAPK) (a), and TGF-β-activated kinase 1 (TAK1) (b) in LPS-activated microglial cells. BV2 cells were pretreated with bee venom or vehicle for 30 min and then incubated with LPS for the indicated times. The protein extraction and protein separation by SDS-PAGE are described in Section 2. β-actin was used as an internal loading control for the immunoblot analysis. Representative images of experiments performed at least in triplicate are shown. versus LPS alone.

(a)

(b)