Evidence-Based Complementary and Alternative Medicine / 2016 / Article / Fig 7

Research Article

Anti-Inflammatory Activity of Bee Venom in BV2 Microglial Cells: Mediation of MyD88-Dependent NF-κB Signaling Pathway

Figure 7

The interaction of MyD88 with either TRAF6 and IKKα/β or MKK4 and TAK1 was diminished by bee venom treatment in LPS-stimulated BV2 microglia. The inhibitory effects of bee venom on the interaction of MyD88 with TRAF6 (a) and IKKα/β or MKK4 and TAK1 (b) in LPS-activated microglial cells are shown. BV2 cells were pretreated with bee venom or vehicle for 30 min and then incubated with LPS for the indicated times. The protein extraction, immunoprecipitation with MyD88 antibody, and protein separation by SDS-PAGE are described in Section 2. Representative images of experiments performed at least in triplicate are shown.

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