The Extract of Chrysanthemum zawadskii var. latilobum Ameliorates Collagen-Induced Arthritis in Mice

Figure 5

Effects of CZ extract on the production of MMP-1 and MMP-3 in CIA mice and RA fibroblast-like synoviocytes. On day 41, CIA mice’s posterior paws were collected and then RT-PCR was performed. (a) Representative images are shown ( for each group) from three independent experiments. (b) Band densities are presented as the mean ± SEM from three separate experiments. and in comparison with the scores of vehicle-treated groups. (c) The effect of CZ extract on the secretion of MMPs was measured in culture media of TNF-α-stimulated RA fibroblast-like synoviocytes by immunoblotting. The cells were activated with TNF-α (20 ng/mL) for 24 hours with and without the addition of CZ extract. Representative images are submitted from three separate experiments. CZ, the extract of Chrysanthemum zawadskii var. latilobum; IND, indomethacin.

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