Decoction and Fermentation of Selected Medicinal Herbs Promote Hair Regrowth by Inducing Hair Follicle Growth in Conjunction with Wnts Signaling

Figure 5

Effect on Wnts (Wnt3, 10a, and 10b) gene expression in hMSCs. (a-b) Cytotoxicity assays were performed in a 96-well plate for 24 h, and the results were expressed as the percent cytotoxicity for identical treatments of HDE and DE (1–1000 μg/mL). For gene expression analysis, cells were seeded on a 6-well plate and treated with the HDE (100 μg/mL) or DE (100 μg/mL) in the presence or absence of 50 μM phorbol myristic acetate (PMA) for 24 h. (c–e) Wnt3, Wnt10a, and Wnt10b mRNA were quantified by fold units using the real-time polymerase chain reaction. Results are expressed as means ± standard deviations from three separate experiments. < 0.05, < 0.01, and < 0.001 versus Ctrl. Ctrl, control group.

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