Gigantol Inhibits Epithelial to Mesenchymal Process in Human Lung Cancer Cells

Figure 4

The effect of gigantol on Slug degradation process. (a) H460 cells were pretreated with a proteasomal inhibitor lactacystin (Lac) 10 μM or lysosomal inhibitor concanamycin A (CMA) 1 μM for an hour before treatment with 20 μM of gigantol for 24 h. Slug expression was analyzed using Western blotting assay. The immunoblot signals were qualified by densitometry. The data represent mean ± SE (). versus untreated control cells versus gigantol treated cells. (b) H460 cells were pretreated with lactacystin (Lac) 10 μM for an hour, and then the pretreated cells were exposed to a presence of gigantol or left untreated for 3 h. The levels of immunocomplexes were analyzed for ubiquitin using anti-ubiquitin antibody. The blot was reprobed with Slug antibody to confirm the equal basal amount of Slug. Immunoblot signals were qualified by densitometry. The data represent mean ± SE (). versus control cells.

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