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Evidence-Based Complementary and Alternative Medicine
Volume 2016 (2016), Article ID 4758734, 8 pages
http://dx.doi.org/10.1155/2016/4758734
Research Article

Estimation of Bioactive Compound, Maslinic Acid by HPTLC, and Evaluation of Hepatoprotective Activity on Fruit Pulp of Ziziphus jujuba Mill. Cultivars in India

Biodiversity and Palaeobiology, Agharkar Research Institute, G.G. Agarkar Road, Pune 411 004, India

Received 1 September 2015; Accepted 3 January 2016

Academic Editor: Carolyn Slupsky

Copyright © 2016 Anagha Rajopadhye and Anuradha S. Upadhye. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Fruits of Ziziphus jujuba Mill. (family: Rhamnaceae), known as Indian jujube or “Ber,” are of potential nutritional and medicinal value. The objectives of the present study were to estimate bioactive compound maslinic acid by HPTLC method and to evaluate in vitro antioxidant and hepatoprotective activity of eight cultivars of Indian jujube. Maslinic acid and the fruit pulp of various cultivars of Indian jujube, namely, Gola, Sannur, Umaran, Mehrun, and Chhuhara, exhibited significantly high antioxidant and hepatoprotective activity. HPTLC-densitometric method was developed for quantification of maslinic acid from fruits of Indian jujube cultivars. The trend of occurrence of maslinic acid in fruits pulp extracts was as follows: Gola > Sannur > Umaran > Mehrun > Chhuhara > Wild > Kadaka > Apple. A significant correlation was shown by maslinic acid content and prevention of oxidative stress induced by CCl4 in liver slice culture cell treated with extract. Maslinic acid along with its other phytoconstituents like phenols, flavonoids, and ascorbic acid may act as a possible therapeutic agent for preventing hepatotoxicity caused by oxidative stress generated due to the prooxidants like CCl4. This is the first report of fruit pulp extracts of Z. jujube cultivars in India and maslinic acid preventing CCl4 induced damage in liver slice culture cell of mice.