Evidence-Based Complementary and Alternative Medicine / 2016 / Article / Tab 2

Research Article

Estimation of Bioactive Compound, Maslinic Acid by HPTLC, and Evaluation of Hepatoprotective Activity on Fruit Pulp of Ziziphus jujuba Mill. Cultivars in India

Table 2

Effect of fruit pulp extracts of eight cultivars of Z. jujuba and maslinic acid in protecting liver cells from CCl4 induced cytotoxicity by ameliorating oxidative stress.

TreatmentLDH units/100 mg tissue wet wt.SOD units/100 mg tissue wet wt.CAT units/100 mg tissue wet wt.GR units/100 mg tissue wet wt.

Control7.83 ± 1.1617.83 ± 1.4715.00 ± 1.410.151 ± 0.012
CCl443.53 ± 1.6356.33 ± 2.2587.5 ± 1.040.498 ± 0.008
AA7.53 ± 1.4717.5 ± 2.1415.33 ± 1.750.150 ± 0.007
100 g/mL UM7.00 ± 2.0917.43 ± 1.7215.65 ± 1.370.156 ± 0.003
100 g/mL CH8.33 ± 1.1617.23 ± 1.4715.83 ± 1.220.159 ± 0.011
100 g/mL GO7.17 ± 1.4717.0 ± 1.4115.67 ± 1.670.158 ± 0.006
100 g/mL SA7.49 ± 1.1317.60 ± 1.5615.66 ± 1.450.153 ± 0.012
100 g/mL KA7.13 ± 0.9817.89 ± 1.2315.78 ± 1.430.155 ± 0.009
100 g/mL AP7.91 ± 1.2317.56 ± 1.4515.06 ± 1.720.153 ± 0.006
100 g/mL ME7.55 ± 1.5617.33 ± 1.7615.78 ± 1.760.158 ± 0.002
100 g/mL WI7.34 ± 1.8917.09 ± 1.4515.03 ± 1.290.156 ± 0.005
10 g/mL MA8.01 ± 1.7617.21 ± 1.3415.34 ± 1.090.154 ± 0.006
CCl4 + 100 g/mL UM19.83 ± 1.2122.17 ± 1.1625.74 ± 2.280.206 ± 0.008
CCl4 + 100 g/mL CH22.74 ± 2.4824.5 ± 0.5428.24 ± 1.090.236 ± 0.008
CCl4 + 100 g/mL GO19.33 ± 1.9820.78 ± 0.6722.77 ± 2.890.213 ± 0.004
CCl4 + 100 g/mL SA27.0 ± 0.8938.17 ± 1.9436.67 ± 0.810.298 ± 0.008
CCl4 + 100 g/mL KA34.33 ± 1.0432.0 ± 0.8944.67 ± 1.030.362 ± 0.008
CCl4 + 100 g/mL AP39.33 ± 0.8148.67 ± 0.8147.83 ± 0.750.386 ± 0.005
CCl4 + 100 g/mL ME21.33 ± 2.4823.5 ± 0.5727.44 ± 1.390.256 ± 0.008
CCl4 + 100 g/mL WI39.33 ± 0.8148.67 ± 0.8147.83 ± 0.750.386 ± 0.055
CCl4 + 10 g/mL MA15.83 ± 1.2119.17 ± 1.1623.74 ± 2.280.216 ± 0.003
CCl4 + 50 mM AA13.67 ± 1.0315.33 ± 1.6319.83 ± 1.830.189 ± 0.018

Cytotoxicity was assessed in terms of % lactate dehydrogenase (LDH) released, and the response to oxidative stress was measured in terms of antioxidant enzymes. SOD: superoxide dismutase; CAT: catalase; GR: glutathione reductase activity. Ascorbic acid (AA) was used as a standard. UM: Umaran; CH: Chhuhar; GO: Gola; SA: Sannur; KA: Kadaka; AP: Apple; ME: Mehrun; WI: Wild; MA: maslinic acid.
Values represent means of at least three experiments and their standard deviation.
Significantly different compared with respective CCl4 treated group, .
Significantly different compared with respective CCl4 treated group, .