Estimation of Bioactive Compound, Maslinic Acid by HPTLC, and Evaluation of Hepatoprotective Activity on Fruit Pulp of Ziziphus jujuba Mill. Cultivars in India
Table 3
Percentage release of LDH and extent of lipid peroxidation in liver slice culture in CCl4 induced cytotoxicity.
Treatment
Time at which activity was measured
0.5 h
1 h
1.5 h
2 h
% LDH release
Control
3.67 ± 0.97
4.33 ± 0.95
5.67 ± 1.12
7.82 ± 1.23
CCl4
29.32 ± 1.34
34.55 ± 1.23
37.78 ± 1.56
43.98 ± 1.28
AA
4.88 ± 0.98
5.09 ± 0.95
7.01 ± 1.16
7.34 ± 1.21
100 g/mL UM
4.23 ± 0.87
5.13 ± 0.66
6.59 ± 1.23
7.67 ± 1.56
100 g/mL CH
4.66 ± 0.97
4.91 ± 0.95
5.77 ± 1.12
7.55 ± 1.23
100 g/mL GO
4.39 ± 1.34
5.60 ± 1.23
6.01 ± 1.56
6.93 ± 1.28
100 g/mL SA
4.02 ± 1.56
5.12 ± 1.56
6.89 ± 1.12
7.08 ± 1.51
100 g/mL KA
4.12 ± 1.34
5.04 ± 0.95
6.45 ± 0.98
7.56 ± 1.34
100 g/mL AP
4.56 ± 1.12
5.45 ± 1.12
6.23 ± 1.58
8.01 ± 1.89
100 g/mL ME
4.78 ± 1.78
6.01 ± 1.23
6.79 ± 1.23
7.34 ± 1.56
100 g/mL WI
4.71 ± 1.23
5.98 ± 1.34
6.90 ± 1.77
8.12 ± 1.43
10 g/mL MA
4.55 ± 1.66
5.78 ± 1.28
6.55 ± 1.56
7.22 ± 1.67
CCl4 + 100 g/mL UM
23.45 ± 1.12
33.71 ± 1.34
25.13 ± 1.34
21.41 ± 1.89
CCl4 + 100 g/mL CH
22.76 ± 1.67
32.17 ± 1.11
24.53 ± 1.44
21.45 ± 1.67
CCl4 + 100 g/mL GO
21.64 ± 0.67
30.37 ± 1.79
24.09 ± 1.12
20.97 ± 1.56
CCl4 + 100 g/mL SA
23.78 ± 1.12
34.88 ± 1.09
27.04 ± 1.15
23.88 ± 1.71
CCl4 + 100 g/mL KA
27.89 ± 1.23
33.56 ± 1.15
27.81 ± 1.12
24.78 ± 1.51
CCl4 + 100 g/mL AP
28.45 ± 0.96
34.01 ± 1.34
28.79 ± 1.15
26.33 ± 1.66
CCl4 + 100 g/mL ME
23.05 ± 1.66
32.44 ± 1.02
25.67 ± 1.08
22.66 ± 1.14
CCl4 + 100 g/mL WI
29.08 ± 0.98
32.16 ± 0.95
28.82 ± 1.12
26.28 ± 1.2
CCl4 + 10 g/mL MA
20.34 ± 1.01
24.55 ± 1.67
19.67 ± 1.23
15.33 ± 1.77
CCl4 + 50 mM AA
18.58 ± 0.97
21.69 ± 0.95
15.27 ± 1.12
13.27 ± 1.23
moles of MDA
Control
1.63 ± 0.14
2.18 ± 0.15
2.23 ± 0.08
2.29 ± 0.18
CCl4
2.31 ± 0.17
2.89 ± 0.15
3.36 ± 0.15
4.02 ± 0.14
AA
1.71 ± 0.18
2.02 ± 0.18
2.03 ± 0.16
2.16 ± 0.14
100 g/mL UM
1.83 ± 0.28
2.01 ± 0.15
2.11 ± 0.17
2.18 ± 0.19
100 g/mL CH
1.69 ± 0.14
2.09 ± 0.21
2.11 ± 0.19
2.16 ± 0.16
100 g/mL GO
1.62 ± 0.13
2.01 ± 0.24
2.13 ± 0.22
2.21 ± 0.24
100 g/mL SA
1.45 ± 0.15
2.11 ± 0.78
2.67 ± 0.34
2.72 ± 0.66
100 g/mL KA
1.72 ± 0.45
2.01 ± 0.77
2.16 ± 0.12
2.23 ± 0.71
100 g/mL AP
1.98 ± 0.79
2.05 ± 0.34
2.25 ± 0.56
2.67 ± 0.51
100 g/mL ME
1.23 ± 0.12
1.67 ± 0.22
2.09 ± 0.14
2.27 ± 0.56
100 g/mL WI
1.71 ± 0.48
1.98 ± 0.56
2.17 ± 0.23
2.87 ± 0.32
10 g/mL MA
1.12 ± 0.44
1.98 ± 0.56
2.05 ± 0.31
2.45 ± 0.28
CCl4 + 100 g/mL UM
2.04 ± 0.09
2.36 ± 0.11
2.85 ± 0.24
2.47 ± 0.28
CCl4 + 100 g/mL CH
2.11 ± 0.15
2.43 ± 0.18
2.94 ± 0.09
2.31 ± 0.14
CCl4 + 100 g/mL GO
2.10 ± 0.89
2.76 ± 0.15
2.91 ± 0.81
2.45 ± 0.66
CCl4 + 100 g/mL SA
2.24 ± 0.51
2.57 ± 0.28
2.91 ± 0.93
2.45 ± 0.48
CCl4 + 100 g/mL KA
2.51 ± 0.12
3.22 ± 0.55
3.49 ± 0.11
3.02 ± 0.78
CCl4 + 100 g/mL AP
2.41 ± 0.12
3.02 ± 0.55
3.31 ± 0.11
2.99 ± 0.78
CCl4 + 100 g/mL ME
2.26 ± 0.45
2.53 ± 0.81
2.97 ± 0.29
2.38 ± 0.19
CCl4 + 100 g/mL WI
2.34 ± 0.22
2.99 ± 0.25
3.22 ± 0.14
3.03 ± 0.18
CCl4 + 10 g/mL MA
1.82 ± 0.48
2.76 ± 0.31
2.81 ± 0.75
2.45 ± 0.56
CCl4 + 50 mM AA
1.72 ± 0.21
2.21 ± 0.19
2.53 ± 0.20
2.25 ± 0.29
CCl4: carbon tetrachloride; AA: standard ascorbic acid, at 50 mM concentration; LPO: lipid peroxidation; LDH: lactate dehydrogenase; UM: Umaran; CH: Chhuhar; GO: Gola; SA: Sannur; KA: Kadaka; AP: Apple; ME: Mehrun; WI: Wild; MA: maslinic acid. Values are mean of three experiments. Significantly different compared with respective CCl4 treated group, . Significantly different compared with respective CCl4 treated group, .
