Evidence-Based Complementary and Alternative Medicine / 2016 / Article / Tab 3

Research Article

Estimation of Bioactive Compound, Maslinic Acid by HPTLC, and Evaluation of Hepatoprotective Activity on Fruit Pulp of Ziziphus jujuba Mill. Cultivars in India

Table 3

Percentage release of LDH and extent of lipid peroxidation in liver slice culture in CCl4 induced cytotoxicity.

TreatmentTime at which activity was measured
0.5 h1 h1.5 h2 h

% LDH releaseControl3.67 ± 0.974.33 ± 0.955.67 ± 1.127.82 ± 1.23
CCl429.32 ± 1.3434.55 ± 1.2337.78 ± 1.5643.98 ± 1.28
AA4.88 ± 0.985.09 ± 0.957.01 ± 1.167.34 ± 1.21
100 g/mL UM4.23 ± 0.875.13 ± 0.666.59 ± 1.237.67 ± 1.56
100 g/mL CH4.66 ± 0.974.91 ± 0.955.77 ± 1.127.55 ± 1.23
100 g/mL GO4.39 ± 1.345.60 ± 1.236.01 ± 1.566.93 ± 1.28
100 g/mL SA4.02 ± 1.565.12 ± 1.566.89 ± 1.127.08 ± 1.51
100 g/mL KA4.12 ± 1.345.04 ± 0.956.45 ± 0.987.56 ± 1.34
100 g/mL AP4.56 ± 1.125.45 ± 1.126.23 ± 1.588.01 ± 1.89
100 g/mL ME4.78 ± 1.786.01 ± 1.236.79 ± 1.237.34 ± 1.56
100 g/mL WI4.71 ± 1.235.98 ± 1.346.90 ± 1.778.12 ± 1.43
10 g/mL MA4.55 ± 1.665.78 ± 1.286.55 ± 1.567.22 ± 1.67
CCl4 + 100 g/mL UM23.45 ± 1.1233.71 ± 1.3425.13 ± 1.3421.41 ± 1.89
CCl4 + 100 g/mL CH22.76 ± 1.6732.17 ± 1.1124.53 ± 1.4421.45 ± 1.67
CCl4 + 100 g/mL GO21.64 ± 0.6730.37 ± 1.7924.09 ± 1.1220.97 ± 1.56
CCl4 + 100 g/mL SA23.78 ± 1.1234.88 ± 1.0927.04 ± 1.1523.88 ± 1.71
CCl4 + 100 g/mL KA27.89 ± 1.2333.56 ± 1.1527.81 ± 1.1224.78 ± 1.51
CCl4 + 100 g/mL AP28.45 ± 0.9634.01 ± 1.3428.79 ± 1.1526.33 ± 1.66
CCl4 + 100 g/mL ME23.05 ± 1.6632.44 ± 1.0225.67 ± 1.0822.66 ± 1.14
CCl4 + 100 g/mL WI29.08 ± 0.9832.16 ± 0.9528.82 ± 1.1226.28 ± 1.2
CCl4 + 10 g/mL MA20.34 ± 1.0124.55 ± 1.6719.67 ± 1.2315.33 ± 1.77
CCl4 + 50 mM AA18.58 ± 0.9721.69 ± 0.9515.27 ± 1.1213.27 ± 1.23

moles of MDA Control1.63 ± 0.142.18 ± 0.152.23 ± 0.082.29 ± 0.18
CCl42.31 ± 0.172.89 ± 0.153.36 ± 0.154.02 ± 0.14
AA1.71 ± 0.182.02 ± 0.182.03 ± 0.162.16 ± 0.14
100 g/mL UM1.83 ± 0.282.01 ± 0.152.11 ± 0.172.18 ± 0.19
100 g/mL CH1.69 ± 0.142.09 ± 0.212.11 ± 0.192.16 ± 0.16
100 g/mL GO1.62 ± 0.132.01 ± 0.242.13 ± 0.222.21 ± 0.24
100 g/mL SA1.45 ± 0.152.11 ± 0.782.67 ± 0.342.72 ± 0.66
100 g/mL KA1.72 ± 0.452.01 ± 0.772.16 ± 0.122.23 ± 0.71
100 g/mL AP1.98 ± 0.792.05 ± 0.342.25 ± 0.562.67 ± 0.51
100 g/mL ME1.23 ± 0.121.67 ± 0.222.09 ± 0.142.27 ± 0.56
100 g/mL WI1.71 ± 0.481.98 ± 0.562.17 ± 0.232.87 ± 0.32
10 g/mL MA1.12 ± 0.441.98 ± 0.562.05 ± 0.312.45 ± 0.28
CCl4 + 100 g/mL UM2.04 ± 0.092.36 ± 0.112.85 ± 0.242.47 ± 0.28
CCl4 + 100 g/mL CH2.11 ± 0.152.43 ± 0.182.94 ± 0.092.31 ± 0.14
CCl4 + 100 g/mL GO2.10 ± 0.892.76 ± 0.152.91 ± 0.812.45 ± 0.66
CCl4 + 100 g/mL SA2.24 ± 0.512.57 ± 0.282.91 ± 0.932.45 ± 0.48
CCl4 + 100 g/mL KA2.51 ± 0.123.22 ± 0.553.49 ± 0.113.02 ± 0.78
CCl4 + 100 g/mL AP2.41 ± 0.123.02 ± 0.553.31 ± 0.112.99 ± 0.78
CCl4 + 100 g/mL ME2.26 ± 0.452.53 ± 0.812.97 ± 0.292.38 ± 0.19
CCl4 + 100 g/mL WI2.34 ± 0.222.99 ± 0.253.22 ± 0.143.03 ± 0.18
CCl4 + 10 g/mL MA1.82 ± 0.482.76 ± 0.312.81 ± 0.752.45 ± 0.56
CCl4 + 50 mM AA1.72 ± 0.212.21 ± 0.192.53 ± 0.202.25 ± 0.29

CCl4: carbon tetrachloride; AA: standard ascorbic acid, at 50 mM concentration; LPO: lipid peroxidation; LDH: lactate dehydrogenase; UM: Umaran; CH: Chhuhar; GO: Gola; SA: Sannur; KA: Kadaka; AP: Apple; ME: Mehrun; WI: Wild; MA: maslinic acid.
Values are mean of three experiments.
Significantly different compared with respective CCl4 treated group, .
Significantly different compared with respective CCl4 treated group, .