Antcin K, a Triterpenoid Compound from Antrodia camphorata, Displays Antidiabetic and Antihyperlipidemic Effects via Glucose Transporter 4 and AMP-Activated Protein Kinase Phosphorylation in Muscles

Figure 2

Effect of antcin K (AnK) on GLUT4, phospho-Akt/total-Akt, and phospho-AMPK/total-AMPK in vitro. C2C12 myoblasts cells were treated with AnK compounds as described in the experimental procedures and equal amounts of lysates were resolved by SDS PAGE and blotted for GLUT4, Akt, phospho-Akt (Ser473), AMPK, and phospho-AMPK (Thr¹⁷²). (a) Representative blots for AnK in C2C12 myoblasts cells; (b) quantification of the GLUT4 protein contents and the ratio of phospho-Akt to total-Akt and phospho-AMPK to total-AMPK. All values are means ± SE. compared with the control group.

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(b)