Antcin K, a Triterpenoid Compound from Antrodia camphorata, Displays Antidiabetic and Antihyperlipidemic Effects via Glucose Transporter 4 and AMP-Activated Protein Kinase Phosphorylation in Muscles

Figure 5

Semiquantitative RT-PCR analysis on G6 Pase, DGAT2, PPARα, SREBP1c, aP2, apoCIII, and SREBP2 mRNA levels in liver tissue of the mice by oral gavage antcin K (AnK1, AnK2, or AnK3, 10, 20, or 40 mg/kg body weight, resp.); Feno, fenofibrate (250 mg/kg body weight); Metf, metformin (300 mg/kg body weight): (a) representative image; (b, c) quantification of the ratio of target gene to GAPDH mRNA expression. Total RNA (1 μg) isolated from tissue was reverse-transcribed by MMLV-RT; 10 μL of RT products was used as templates for PCR. The expression levels of G6 Pase, DGAT2, PPARα, SREBP1c, aP2, apoCIII, and SREBP2 mRNA were measured and quantified by image analysis. Values were normalized to GAPDH mRNA expression. All values are means ± SE (). compared with the control (CON) group; , , and compared with the high-fat-diet plus vehicle (distilled water) (HF) group.

(a)

(b)

(c)