The Induction of Apoptosis in A375 Malignant Melanoma Cells by Sutherlandia frutescens

Figure 10

AIF translocates to the nucleus in response to S. frutescens extract. AIF is localized in the mitochondria of control-treated cells. S. frutescens treatment induces the nuclear translocation of AIF, as indicated by white arrows. Z-VAD-fmk does not prevent the nuclear translocation of AIF in response to S. frutescens. Cells were stained with Hoechst 33258 to label the nuclei and Alexa-tagged anti-AIF antibody to determine the subcellular localization of AIF. The white arrows indicate the nuclear localization of AIF. The scale bar represents 20 μm.