The Induction of Apoptosis in A375 Malignant Melanoma Cells by Sutherlandia frutescens

Figure 3

S. frutescens extract induces apoptosis in A375 cells. Representative scatterplots of annexin V-FITC and PI for control-treated cells (a)–(c), cells treated with S. frutescens (d)–(f), cells treated with etoposide, and a known inducer of apoptosis (g)–(i) for 24, 48, and 72 h as well as cells treated with hydrogen peroxide for 30 min, which served as a necrotic control (j). The percentage of the cell population ± the SEM () that is in Q1 (necrotic), Q2 (late apoptotic), Q3 (viable), or Q4 (early apoptotic) is shown. A significant difference compared to the control-treated cells is indicated by ().

(a) Control (24 h)

(b) Control (48 h)

(c) Control (72 h)

(d) S. frutescens (24 h)

(e) S. frutescens (48 h)

(f) S. frutescens (72 h)

(g) Etoposide (24 h)

(h) Etoposide (48 h)

(i) Etoposide (72 h)

(j) H₂O₂ (30 min)