The Induction of Apoptosis in A375 Malignant Melanoma Cells by Sutherlandia frutescens

Figure 5

S. frutescens extract causes caspase activation. The activity of (a) caspase 8, (b) caspase 9, and (c) caspase 3/7 in control- and S. frutescens-treated A375 cells was measured using the Caspase-Glo assays 24, 48, and 72 h after treatment. The activity of the caspases in the S. frutescens-treated cells is shown as a fold increase relative to the control-treated cells. Error bars represent the SEM () and indicates a significant difference compared to the control-treated cells (). (d) Cleaved PARP was detected by western blot and confirmed the activity of the executioner caspases in response to S. frutescens treatment. Etoposide treatment served as a positive apoptotic control and β-actin served as a loading control.

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