The Induction of Apoptosis in A375 Malignant Melanoma Cells by Sutherlandia frutescens

Figure 8

Morphology of A375 cells, pretreated with Z-VAD-fmk, in response to control or S. frutescens treatment after 24, 48, or 72 h. The pretreatment with Z-VAD-fmk did not alter the morphology of the control-treated cells (a)–(c) indicating that it is not cytotoxic. Z-VAD-fmk did not prevent cell detachment in response to S. frutescens extract (d)–(f). Scale bar represents 20 μm.

(a) Control + Z-VAD-fmk (24 h)

(b) Control + Z-VAD-fmk (48 h)

(c) Control + Z-VAD-fmk (72 h)

(d) S. frutescens + Z-VAD-fmk (24 h)

(e) S. frutescens + Z-VAD-fmk (48 h)

(f) S. frutescens + Z-VAD-fmk (72 h)