Serum Protein KNG1, APOC3, and PON1 as Potential Biomarkers for Yin-Deficiency-Heat Syndrome

Table 1

Upregulated serum proteins in YDH syndrome group quantified by iTRAQ-based proteomics and the ratios to the control group.

Protein ID	Gene name	Protein name	M/C (114/113)			M/C (116/115)
			Run 1	Run 2	Mean	Run 1	Run 2	Mean
P62161	Calm1	Calmodulin	8.09	16.75	12.42	12.82	6.08	9.45
P62260	Ywhae	14-3-3 protein epsilon	2.68	6.31	4.49	15.28	14.59	14.93
Q6AYZ1	Tuba1c	Tubulin alpha-1C chain	9.29	4.79	7.04	3.91	17.22	10.56
P45592	Cfl1	Cofilin-1	4.37	3.44	3.90	11.07	13.93	12.50
P01048	Map1	T-kininogen 1	5.50	4.57	5.03	16.29	5.65	10.97
P09006	Serpina3n	Serine protease inhibitor A3N	2.99	2.86	2.92	11.91	13.18	12.55
P08932	—	T-kininogen 2	3.91	4.79	4.35	14.72	6.14	10.43
Q62812	Myh9	Myosin-9	7.11	9.55	8.33	6.79	6.03	6.41
P18418	Calr	Calreticulin	4.13	2.75	3.44	8.55	9.73	9.14
Q66HD0	Hsp90b1	Endoplasmin	3.02	6.25	4.64	13.55	2.03	7.79
P34058	Hsp90ab1	Heat shock protein HSP 90-beta	4.25	9.64	6.94	5.25	5.50	5.37
P68511	Ywhah	14-3-3 protein eta	3.84	1.46	2.65	13.68	5.06	9.37
P04276	Gc	Vitamin D-binding protein	6.61	4.45	5.53	5.65	6.03	5.84
Q62636	Rap1b	Ras-related protein Rap-1b	3.19	2.58	2.89	8.71	7.66	8.18
Q9Z1P2	Actn1	Alpha-actinin-1	2.99	2.63	2.81	6.79	8.79	7.79
P09495	Tpm4	Tropomyosin alpha-4 chain	2.81	2.51	2.66	6.14	9.12	7.63
Q6PCT3	Tpd52l2	Tumor protein D54	1.71	6.31	4.01	4.41	7.87	6.14
P11980	Pkm	Pyruvate kinase isozymes M1/M2	1.82	2.56	2.19	11.27	4.57	7.92
Q08163	Cap1	Adenylyl cyclase-associated protein 1	3.22	3.94	3.58	4.17	8.39	6.28
P11598	Pdia3	Protein disulfide-isomerase A3	11.17	3.16	7.17	2.13	2.27	2.20
P16086	Sptan1	Spectrin alpha chain	2.38	3.16	2.77	5.92	6.92	6.42
P62963	Pfn1	Profilin-1	3.13	2.31	2.72	4.79	7.45	6.12
Q68FR2	Bin2	Bridging integrator 2	2.25	2.17	2.21	6.03	6.08	6.05
Q63610	Tpm3	Tropomyosin alpha-3 chain	2.21	2.70	2.46	5.30	6.25	5.77
P06866	Hp	Haptoglobin	7.11	3.50	5.31	2.75	3.05	2.90
P08934	Kng1	Kininogen-1	5.86	4.88	5.37	1.60	2.83	2.22
B0BNA5	Cotl1	Coactosin-like protein	2.56	2.25	2.40	5.65	4.53	5.09
Q63081	Pdia6	Protein disulfide-isomerase A6	3.02	4.21	3.61	4.29	3.44	3.86
P85972	Vcl	Vinculin	3.56	3.08	3.32	4.09	3.94	4.02
P04642	Ldha	L-lactate dehydrogenase A chain	4.41	1.67	3.04	6.61	1.82	4.21
P63102	Msfs1	14-3-3 protein zeta/delta	2.81	1.84	2.32	5.20	4.53	4.86
B2GUZ5	Capza1	F-actin-capping protein subunit alpha-1	2.23	1.72	1.98	3.73	6.49	5.11
P46462	Vcp	Transitional endoplasmic reticulum ATPase	2.99	2.40	2.70	2.65	5.45	4.05
P20767	—	Ig lambda-2 chain C region	2.25	2.05	2.15	2.58	6.55	4.56
Q5XFX0	Tagln2	Transgelin-2	3.63	2.07	2.85	2.81	4.37	3.59
Q4V7E8	Lrrfip2	Flightless-interacting protein 2	2.33	1.96	2.15	4.06	3.98	4.02
Q63617	Hyou1	Hypoxia upregulated protein 1	1.79	5.97	3.88	1.75	2.58	2.17
Q62930	C9	Complement component C9	3.63	2.83	3.23	2.25	3.25	2.75
Q5XI73	Arhgdia	Rho GDP-dissociation inhibitor 1	1.74	2.99	2.37	2.96	4.02	3.49
P04797	Gapdh	Dehydrogenase	1.77	1.51	1.64	3.50	4.92	4.21
Q07009	Capn2	Calpain-2 catalytic subunit	1.28	3.60	2.44	3.80	2.65	3.23
Q5U211	Snx3	Sorting nexin-3	1.91	4.25	3.08	1.60	3.22	2.41
Q63514	C4bpa	C4b-binding protein alpha chain	2.99	2.49	2.74	2.88	2.31	2.60
Q64119	Myl6	Myosin light polypeptide 6	1.72	2.27	2.00	3.60	2.68	3.14
P97571	Capn1	Calpain-1 catalytic	3.73	2.15	2.94	2.11	1.61	1.86
P20760	Igg-2a	Ig gamma-2A chain C region	1.50	1.84	1.67	2.63	3.22	2.93
P06761	Hspa5	78 kDa glucose-regulated protein	1.56	1.46	1.51	3.53	2.56	3.05
Q63515	C4bpb	C4b-binding protein beta chain	3.60	2.09	2.84	1.56	1.61	1.59
Q7M0E3	Dstn	Destrin	3.16	1.29	2.23	2.61	1.58	2.10
Q9EPH8	Pabpc1	Polyadenylate-binding protein 1	1.41	1.80	1.60	3.40	2.00	2.70
P63018	Hspa8	Heat shock cognate 71 kDa protein	1.60	2.19	1.89	2.63	2.17	2.40
P04785	P4hb	Protein disulfide-isomerase	2.25	2.01	2.13	1.82	2.49	2.15
Q9EQX9	Ube2n	Ubiquitin-conjugating enzyme E2 N	2.86	1.58	2.22	1.87	2.15	2.01
P06399	Fga	Fibrinogen alpha chain	4.37	1.43	2.90	1.33	1.28	1.31
P01835	—	Ig kappa chain C region	2.01	1.57	1.79	2.03	2.70	2.37
P12346	Tf	Serotransferrin	1.60	1.91	1.75	2.54	2.03	2.28
Q9JLT6	Bid	BH3-interacting domain death agonist	1.36	1.64	1.50	1.69	3.05	2.37
P68101	Eif2s1	Eukaryotic translation initiation factor	1.66	1.26	1.46	3.16	1.46	2.31
P35213	Ywhab	14-3-3 protein beta/alpha	1.85	1.28	1.57	2.65	1.49	2.07
Q91ZN1	Coro1a	Coronin-1A	1.64	1.37	1.51	2.36	1.69	2.02
P04906	Gstp1	Glutathione S-transferase P	1.33	1.47	1.40	2.51	1.67	2.09
P14272	Klkb1	Plasma kallikrein	2.11	1.96	2.03	1.27	1.42	1.34
P07335	Ckb	Creatine kinase B-type	1.41	1.28	1.34	1.57	1.38	1.48

M, model group; C, control group; 114/113 and 116/115, two biological replicates; Run 1 and Run 2, two technical replicates.