Figure 1: Morphology and immunofluorescence in RCECs. RCECs from rat retinal microvascular fragments were cultured in endothelial cell medium. When the RCECs were cultured for 10 days, the characteristic cobble-stone morphology of RCECs was observed (a). The RCECs were positive for both vWf (b, green fluorescent) and CD31 (c, green fluorescent), as determined by an immunofluorescent assay. All nuclei were stained with DAPI (blue fluorescent).