Effects of Cymbidium Root Ethanol Extract on Atopic Dermatitis

Figure 1

Effect of Cymbidium ethanol extract (CYM) on immunoglobulin E/dinitrophenyl-conjugated bovine serum albumin- (IgE/DNP-BSA-) induced passive cutaneous anaphylaxis. Anti-DNP IgE antibody was injected intradermally into each right ear of 7-week-old ICR mice. CYM or diphenhydramine (DPH) was administered orally to mice. After 1 h, we injected 200 μg of DNP-BSA. (a) Representative images of ears. (b) Excised ears were reacted overnight with 500 μL of formamide and amount of dye taken up was determined by measuring absorbance at 620 nm using a microplate reader. Data are mean ± standard deviation (SD, ); versus IgE/DNP-BSA treatment group.

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