Effects of Cymbidium Root Ethanol Extract on Atopic Dermatitis

Figure 4

Effect of Cymbidium ethanol extract (CYM) on 2,4-dinitrochlorobenzene- (DNCB-) induced expression of interleukin- (IL-) 4, IL-13, and tumor necrosis factor- (TNF-) mRNA in atopic dermatitis- (AD-) like mouse model. After inducing AD, 10 mg/mL CYM solution (in 3 : 1 mixture of acetone and olive oil) was applied to the dorsal skin of mice for a total of 6 times over a 2-week period. After euthanasia, dorsal skin lesions were enucleated from mice of all three groups (non-, DNCB, DNCB, and CYM treatment). Total RNA was isolated from extracted tissue of dorsal lesions using Tri-reagent. Total RNA was used as a template for cDNA synthesis, which was performed using a cDNA Synthesis kit. Real-time polymerase chain reaction (qPCR) analysis was carried out using SYBR Green I and a Lightcycler 96 instrument. The qPCR analysis was performed to detect IL-4, IL-13, and TNF-α mRNA expression. Data are mean ± standard deviation (SD, ). versus DNCB treatment.