Effects of Cymbidium Root Ethanol Extract on Atopic Dermatitis

Figure 5

Effect of Cymbidium ethanol extract (CYM) on viability and immunoglobulin E/dinitrophenyl-conjugated bovine serum albumin- (IgE/DNP-BSA-) induced degranulation of RBL-2H3 cells. RBL-2H3 cells were seeded and incubated in 24-well plates overnight in Eagle’s Minimal Essential Medium (MEM). To sensitize RBL-2H3 cells, anti-DNP IgE (400 ng/mL) was added to culture medium. After 12 h, RBL-2H3 cells were washed with Siraganian buffer and treated with CYM (0, 25, 50, 100, or 200 μg/mL) in Siraganian buffer for 30 min. DNP-BSA (50 ng/mL) was added for 15 min. Cell supernatants were transferred to a 96-well plate, mixed with 1 mM p-NAG in 0.1 M citrate buffer for 1 h, and absorbance was measured at 405 nm using a microplate reader. Data are mean ± standard deviation (SD, ). and versus sensitized RBL-2H3 cells.