Effects of Cymbidium Root Ethanol Extract on Atopic Dermatitis

Figure 6

Effect of Cymbidium ethanol extract (CYM) on immunoglobulin E/dinitrophenyl-conjugated bovine serum albumin- (IgE/DNP-BSA-) induced phosphorylation of spleen tyrosine kinase (Syk), phospholipase C- (PLC-) γ, Akt, extracellular signal-regulated kinase (Erk) 1/2, c-Jun N-terminal kinase (JNK), and p38. RBL-2H3 cells were seeded and incubated in 24-well plates overnight in Minimum Essential Medium (MEM). Anti-DNP IgE (400 ng/mL) was added for 12 h, followed by addition of CYM (0 or 200 μg/mL). Cells were then treated with DNP-BSA. Cells were lysed and protein was extracted by the PRO-PREP protein extraction kit. The protein lysates were analyzed by SDS-PAGE. (a) Western blot analysis for phosphorylation of Syk, PLC-γ, Akt, Erk1/2, JNK, and p38. (b) Densitometric analysis of expression of phosphorylated proteins. Data are mean ± standard deviation (SD, ); and versus sensitized RBL-2H3 cell.

(a)

(b)