Table of Contents Author Guidelines Submit a Manuscript
Evidence-Based Complementary and Alternative Medicine
Volume 2016, Article ID 5815946, 12 pages
http://dx.doi.org/10.1155/2016/5815946
Research Article

Naoxintong Protects Primary Neurons from Oxygen-Glucose Deprivation/Reoxygenation Induced Injury through PI3K-Akt Signaling Pathway

1Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
2Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
3Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China
4School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China

Received 25 November 2015; Accepted 24 December 2015

Academic Editor: Ki-Wan Oh

Copyright © 2016 Yan Ma et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. E. H. Lo, T. Dalkara, and M. A. Moskowitz, “Mechanisms, challenges and opportunities in stroke,” Nature Reviews Neuroscience, vol. 4, no. 5, pp. 399–415, 2003. View at Publisher · View at Google Scholar · View at Scopus
  2. W. Hacke, M. Kaste, E. Bluhmki et al., “Thrombolysis with alteplase 3 to 4.5 hours after acute ischemic stroke,” The New England Journal of Medicine, vol. 359, no. 13, pp. 1317–1329, 2008. View at Publisher · View at Google Scholar · View at Scopus
  3. Y. Wang, X. Liao, X. Zhao et al., “Using recombinant tissue plasminogen activator to treat acute ischemic stroke in China: analysis of the results from the Chinese National Stroke Registry (CNSR),” Stroke, vol. 42, no. 6, pp. 1658–1664, 2011. View at Publisher · View at Google Scholar · View at Scopus
  4. B. Wu, M. Liu, H. Liu et al., “Meta-analysis of traditional Chinese patent medicine for ischemic stroke,” Stroke, vol. 38, no. 6, pp. 1973–1979, 2007. View at Publisher · View at Google Scholar · View at Scopus
  5. G. Cai, B. Liu, W. Liu et al., “Buyang Huanwu Decoction can improve recovery of neurological function, reduce infarction volume, stimulate neural proliferation and modulate VEGF and Flk1 expressions in transient focal cerebral ischaemic rat brains,” Journal of Ethnopharmacology, vol. 113, no. 2, pp. 292–299, 2007. View at Publisher · View at Google Scholar · View at Scopus
  6. H. Zhang, W.-R. Wang, R. Lin et al., “Buyang Huanwu decoction ameliorates coronary heart disease with Qi deficiency and blood stasis syndrome by reducing CRP and CD40 in rats,” Journal of Ethnopharmacology, vol. 130, no. 1, pp. 98–102, 2010. View at Publisher · View at Google Scholar · View at Scopus
  7. L. Su, Y. Li, B. Lv et al., “Clinical study on naoxintong capsule for stroke recovery of Qi-deficiency and blood-stasis syndrome,” Zhongguo Zhong Yao Za Zhi, vol. 36, no. 11, pp. 1530–1533, 2011. View at Google Scholar
  8. L. X. Li, L. Chen, and H. J. Zhao, “Effect of naoxintong capsule on the vascular endothelial function and the infarct size of patients with acute myocardial infarction,” Zhongguo Zhong Xi Yi Jie He Za Zhi, vol. 31, no. 12, pp. 1615–1618, 2011. View at Google Scholar
  9. S.-R. Li, T.-H. Wang, and B.-J. Zhang, “Effects of naoxintong capsule on the inflammation and prognosis in borderline lesion coronary heart disease patients,” Zhongguo Zhong Xi Yi Jie He Za Zhi, vol. 32, no. 5, pp. 607–611, 2012. View at Google Scholar · View at Scopus
  10. F. Zhang, B. Huang, Y. Zhao et al., “BNC protects H9c2 cardiomyoblasts from H2O2-induced oxidative injury through ERK1/2 signaling pathway,” Evidence-Based Complementary and Alternative Medicine, vol. 2013, Article ID 802784, 12 pages, 2013. View at Publisher · View at Google Scholar · View at Scopus
  11. M. Liu, Q. Pan, Y. Chen et al., “NaoXinTong inhibits the development of diabetic retinopathy in db/db mice,” Evidence-Based Complementary and Alternative Medicine, vol. 2015, Article ID 242517, 8 pages, 2015. View at Publisher · View at Google Scholar
  12. X.-H. Zhu, J.-M. Yang, and T.-M. Gao, “Neuroprotective effects of Naoxintong against neuronal injury in hippocampal CA1 region following transient forebrain ischemia in rats,” Di Yi Jun Yi Da Xue Xue Bao, vol. 24, no. 10, pp. 1123–1125, 2004. View at Google Scholar · View at Scopus
  13. J. Zhao, H. Zhu, S. Wang et al., “Naoxintong protects against atherosclerosis through lipid-lowering and inhibiting maturation of dendritic cells in LDL receptor knockout mice fed a high-fat diet,” Current Pharmaceutical Design, vol. 19, no. 33, pp. 5891–5896, 2013. View at Publisher · View at Google Scholar · View at Scopus
  14. J. Mei, B. Zhang, and R. Lu, “A Chinese herbal compound induces neurotrophy in astrocyte by a new study method called ‘cerebrospinal fluid pharmacology in Chinese materia medica’,” Zhong Yao Cai, vol. 23, no. 8, pp. 467–470, 2000. View at Google Scholar · View at Scopus
  15. Y.-Q. Wu, Y.-W. Zhou, X.-D. Qin, S.-Y. Hua, Y.-L. Zhang, and L.-Y. Kang, “Cerebrospinal fluid pharmacology: an improved pharmacology approach for chinese herbal medicine research,” Evidence-Based Complementary and Alternative Medicine, vol. 2013, Article ID 674305, 10 pages, 2013. View at Publisher · View at Google Scholar
  16. C. Yan, J. Zhu, X. Jia, C. Wang, S. Wang, and L. Kang, “Panax notoginseng saponin attenuates hypoxia/reoxygenation-induced oxidative stress in cortical neurons,” Neural Regeneration Research, vol. 7, no. 36, pp. 2853–2859, 2012. View at Publisher · View at Google Scholar
  17. Z. Yu, J. Liu, S. Guo et al., “Neuroglobin-overexpression alters hypoxic response gene expression in primary neuron culture following oxygen glucose deprivation,” Neuroscience, vol. 162, no. 2, pp. 396–403, 2009. View at Publisher · View at Google Scholar · View at Scopus
  18. G. Hajnóczky, L. D. Robb-Gaspers, M. B. Seitz, and A. P. Thomas, “Decoding of cytosolic calcium oscillations in the mitochondria,” Cell, vol. 82, no. 3, pp. 415–424, 1995. View at Publisher · View at Google Scholar · View at Scopus
  19. H. Wei, P. Leeds, R.-W. Chen et al., “Neuronal apoptosis induced by pharmacological concentrations of 3-hydroxykynurenine: characterization and protection by dantrolene and Bcl-2 overexpression,” Journal of Neurochemistry, vol. 75, no. 1, pp. 81–90, 2000. View at Publisher · View at Google Scholar · View at Scopus
  20. X. X. Peng, S. H. Zhang, X. L. Wang et al., “Panax Notoginseng Flower Saponins (PNFS) inhibit LPS-stimulated NO overproduction and iNOS gene overexpression via the suppression of TLR4-mediated MAPK/NF-kappa B signaling pathways in RAW264.7 macrophages,” Chinese Medicine, vol. 10, article 15, 2015. View at Publisher · View at Google Scholar
  21. F. Zhang, J. Cui, B. Lv, and B. Yu, “Nicorandil protects mesenchymal stem cells against hypoxia and serum deprivation-induced apoptosis,” International Journal of Molecular Medicine, vol. 36, no. 2, pp. 415–423, 2015. View at Publisher · View at Google Scholar
  22. W.-L. Zhang, Y.-L. Zhao, X.-M. Liu, J. Chen, and D. Zhang, “Protective role of mitochondrial K-ATP channel and mitochondrial membrane transport pore in rat kidney ischemic postconditioning,” Chinese Medical Journal, vol. 124, no. 14, pp. 2191–2195, 2011. View at Publisher · View at Google Scholar · View at Scopus
  23. N. Noshita, A. Lewén, T. Sugawara, and P. H. Chan, “Evidence of phosphorylation of Akt and neuronal survival after transient focal cerebral ischemia in mice,” Journal of Cerebral Blood Flow and Metabolism, vol. 21, no. 12, pp. 1442–1450, 2001. View at Google Scholar · View at Scopus
  24. P. Lipton, “Ischemic cell death in brain neurons,” Physiological Reviews, vol. 79, no. 4, pp. 1431–1568, 1999. View at Google Scholar · View at Scopus
  25. G. Morciano, C. Giorgi, M. Bonora et al., “Molecular identity of the mitochondrial permeability transition pore and its role in ischemia-reperfusion injury,” Journal of Molecular and Cellular Cardiology, vol. 78, pp. 142–153, 2015. View at Publisher · View at Google Scholar · View at Scopus
  26. Y. Gouriou, N. Demaurex, P. Bijlenga, and U. De Marchi, “Mitochondrial calcium handling during ischemia-induced cell death in neurons,” Biochimie, vol. 93, no. 12, pp. 2060–2067, 2011. View at Publisher · View at Google Scholar · View at Scopus
  27. P. S. Brookes and V. M. Darley-Usmar, “Role of calcium and superoxide dismutase in sensitizing mitochondria to peroxynitrite-induced permeability transition,” The American Journal of Physiology—Heart and Circulatory Physiology, vol. 286, no. 1, pp. H39–H46, 2004. View at Google Scholar · View at Scopus
  28. D. J. Hausenloy, S.-B. Ong, and D. M. Yellon, “The mitochondrial permeability transition pore as a target for preconditioning and postconditioning,” Basic Research in Cardiology, vol. 104, no. 2, pp. 189–202, 2009. View at Publisher · View at Google Scholar · View at Scopus
  29. J. A. Dykens, “Isolated cerebral and cerebellar mitochondria produce free radicals when exposed to elevated Ca2+ and Na+: implications for neurodegeneration,” Journal of Neurochemistry, vol. 63, no. 2, pp. 584–591, 1994. View at Google Scholar · View at Scopus
  30. M. R. Duchen, “Mitochondria and Ca2+ in cell physiology and pathophysiology,” Cell Calcium, vol. 28, no. 5-6, pp. 339–348, 2000. View at Publisher · View at Google Scholar · View at Scopus
  31. O. Kann and R. Kovács, “Mitochondria and neuronal activity,” The American Journal of Physiology—Cell Physiology, vol. 292, no. 2, pp. C641–C657, 2007. View at Publisher · View at Google Scholar · View at Scopus
  32. M. Matsumoto-Ida, M. Akao, T. Takeda, M. Kato, and T. Kita, “Real-time 2-photon imaging of mitochondrial function in perfused rat hearts subjected to ischemia/reperfusion,” Circulation, vol. 114, no. 14, pp. 1497–1503, 2006. View at Publisher · View at Google Scholar · View at Scopus
  33. B. M. Polster and G. Fiskum, “Mitochondrial mechanisms of neural cell apoptosis,” Journal of Neurochemistry, vol. 90, no. 6, pp. 1281–1289, 2004. View at Publisher · View at Google Scholar · View at Scopus
  34. B. M. Polster, K. W. Kinnally, and G. Fiskum, “BH3 death domain peptide induces cell type-selective mitochondrial outer membrane permeability,” The Journal of Biological Chemistry, vol. 276, no. 41, pp. 37887–37894, 2001. View at Google Scholar · View at Scopus
  35. H. Zou, W. J. Henzel, X. Liu, A. Lutschg, and X. Wang, “Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3,” Cell, vol. 90, no. 3, pp. 405–413, 1997. View at Publisher · View at Google Scholar · View at Scopus
  36. L. Lin, H. Chen, Y. Zhang et al., “IL-10 protects neurites in oxygen-glucose-deprived cortical neurons through the PI3K/Akt pathway,” PLoS ONE, vol. 10, no. 9, Article ID e0136959, 2015. View at Publisher · View at Google Scholar
  37. H. Kitagawa, H. Warita, C. Sasaki et al., “Immunoreactive Akt, PI3-K and ERK protein kinase expression in ischemic rat brain,” Neuroscience Letters, vol. 274, no. 1, pp. 45–48, 1999. View at Publisher · View at Google Scholar · View at Scopus
  38. D. C. Henshall, T. Araki, C. K. Schindler et al., “Activation of Bcl-2-associated death protein and counter-response of Akt within cell populations during seizure-induced neuronal death,” Journal of Neuroscience, vol. 22, no. 19, pp. 8458–8465, 2002. View at Google Scholar · View at Scopus
  39. S. G. Kennedy, E. S. Kandel, T. K. Cross, and N. Hay, “Akt/protein kinase B inhibits cell death by preventing the release of cytochrome c from mitochondria,” Molecular and Cellular Biology, vol. 19, no. 8, pp. 5800–5810, 1999. View at Publisher · View at Google Scholar · View at Scopus
  40. H. Zhou, X.-M. Li, J. Meinkoth, and R. N. Pittman, “Akt regulates cell survival and apoptosis at a postmitochondrial level,” Journal of Cell Biology, vol. 151, no. 3, pp. 483–494, 2000. View at Publisher · View at Google Scholar · View at Scopus
  41. W. Songsong, X. Haiyu, M. Yan et al., “Characterization and rapid identification of chemical constituents of NaoXinTong capsules by UHPLC-linear ion trap/Orbitrap mass spectrometry,” Journal of Pharmaceutical and Biomedical Analysis, vol. 111, pp. 104–118, 2015. View at Publisher · View at Google Scholar