Figure 5: Effect of quercetin and Gelam honey extract on TNF alpha expression. Quantitative analysis and representative western blot analysis of TNF alpha in HIT-T15 cells pretreated with quercetin and honey extract in cells cultured in 20 mM ((a), (c), and (e)) and 50 mM ((b), (d), and (f)) glucose. The results were normalized with β-actin antibody. Data were analyzed with one-way ANOVA using SPSS version 16.0 software and are presented as the mean ± standard deviation and represent the mean of three different experiments. (e) and , quercetin and honey extract treated compared to the 20 mM glucose alone. (f) and , quercetin and honey extract treated compared to the 50 mM glucose alone.