Figure 7: Effect of quercetin and Gelam honey extract on pAkt (Ser473) expression. Quantitative analysis and representative western blot analysis of pAkt (Ser473) in HIT-T15 cells pretreated with quercetin and honey extract in cells cultured in 20 mM ((a), (c), and (e)) and 50 mM ((b), (d), and (f)) glucose. An increase in the level of pAkt (Ser473) was observed after pretreatment with quercetin and honey extract. Akt inhibitor VIII prevented the expression of Akt Ser473 phosphorylation induced by quercetin and honey extract. The results were normalized with β-actin antibody. Data were analyzed with one-way ANOVA using SPSS version 16.0 software and are presented as the mean ± standard deviation and represent the mean of three different experiments. (e) and , quercetin and honey extract treated compared to the 20 mM glucose alone. (f) and , quercetin and honey extract treated compared to the 50 mM glucose alone.