Supercritical Fluid Extract of Spent Coffee Grounds Attenuates Melanogenesis through Downregulation of the PKA, PI3K/Akt, and MAPK Signaling Pathways

Figure 3

The effects of spent coffee grounds SFE on melanogenesis-related protein expression and signaling pathways. ((a), (c)) Western blotting of cellular proteins in B16F10 cells. ((b), (d)) The relative amounts of MITF, tyrosinase, MC1R, TRP-1, and TRP-2 or phosphorylated proteins (p-p38, p-JNK, p-ERK, and p-CREB) compared to the total GAPDH were calculated and analyzed using Multi Gauge 3.0 software, and the values represented the mean of triplicate experiments ± standard deviations. .

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