Evidence-Based Complementary and Alternative Medicine

Research Article

A Bioactivity-Based Method for Screening, Identification of Lipase Inhibitors, and Clarifying the Effects of Processing Time on Lipase Inhibitory Activity of Polygonum Multiflorum

UHPLC/Q-TOF-MS identification of lipase inhibitors and IC₅₀ values.


Number	Rt (min)	MS	MS/MS	ppm	Formula	Compound	IC₅₀ (g/mL)

1	1.409	169.0141		4.6	C₇H₆O₅	Gallic acid	—
2	3.725	289.0724	215.0714, 173.0535, 149.0206, 125.0215, 109.0283	2.2	C₁₅H₁₄O₆	Catechin	—
3	4.48	289.0724	276.4677, 205.0499, 163.0368, 131.0053, 109.0317	2.2	C₁₅H₁₄O₆	Epicatechin	—
4	6.484	389.1168	227.1165	3.89	C₂₀H₂₂O₈	Polydatin	1318
5	6.766	405.1184	243.0656, 137.0237	0.95	C₂₀H₂₂O₉	2,3,5,4′-Tetrahydroxystilbene-2-O--D-glucoside	350.5
6	9.203	227.2002	202.0698, 176.0559, 99.9223, 91.0179, 73.5614	3.35	C₁₄H₁₂O₃	Resveratrol	564.3
7	10.597	431.1386	269.0448, 225.0540	0.86	C₂₁H₂₀O₁₀	Emodin-8-O-glucoside	296.0
8	11.836	445.0595	283.0595, 240.0415	0.76	C₂₂H₂₂O₁₀	Physcion-8-O-glucoside	421.5
9	12.127	283.0606	240.0409	3.76	C₁₅H₈O₆	Rhein	231.0
10	17.188	269.0460	241.0491, 225.0549, 210.0312, 197.0598	3.89	C₁₅H₁₀O₅	Emodin	349.7
11	18.786	283.0610	240.0419, 116.9278	0.88	C₁₆H₁₂O₅	Physcion	812.8