Activation of p53/miR-34a Tumor Suppressor Axis by Chinese Herbal Formula JP-1 in A549 Lung Adenocarcinoma Cells

Figure 4

JP-1 induces p53 and its downstream p21 and BAX proteins accompanied by the activation of PARP and caspases in A549 cells. (a) JP-1 increased the p53 protein in a time- and dose-dependent manner. The numbers under the p53 bands show the relative densitometric ratios of p53 to GAPDH bands. (b) Similar time- and dose-dependent increase of p21 protein was observed after treatment with JP-1. (c) JP-1 increased the BAX protein after 24 h of treatment. (d) JP-1 increased the cleaved forms of PARP, caspase-9 and caspase-3 proteins and decreased the full length caspase-8 protein after 48 h of treatment. Cell lysates were analyzed by Western blot, using GAPDH as the loading control.

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