Ethyl Acetate Fraction of Amomum xanthioides Exerts Antihepatofibrotic Actions via the Regulation of Fibrogenic Cytokines in a Dimethylnitrosamine-Induced Rat Model

Figure 3

Total flavonoid and phenolic contents and mRNA expression and protein levels in HSC-T6. (a) Total flavonoid contents values are expressed in terms of quercetin equivalent (mg/g of dry mass) and (b) the total phenolic contents values are expressed in terms of gallic acid equivalent (mg/g of dry mass). The analysis of mRNA expression levels was performed for (c) Col 1a1 and (d) α-SMA using real-time PCR in HSC-T6 cells. (e) The protein levels of Col 1 and α-SMA are examined using western blot. The data are expressed as the mean ± SD (). , compared to the control group; , compared to the TGF-β1 treatment group; comparison of WAX 50 and EFAX 50; , comparison of MFAX 50 and EFAX 50.

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