Methanol and Butanol Extracts of Paeonia lutea Leaves Repress Metastasis of Squamous Cell Carcinoma

Figure 5

Effects of P. lutea leaf extracts on mouse survival and tumor growth in SAS-cell-xenograft nude mice. One million SAS cells were subcutaneously injected into the dorsal flank of nude mice (see Section 2). After 7 d, the mice were divided into 6 groups: vehicle (black lines in (a), (b), and (c) and control in (d)) methanol (red lines and ME), ethyl acetate (blue lines and EA), butanol (yellow lines and BU), butanol-insoluble (green lines and BW), and water (orange lines and W) extracts of P. lutea leaves were administrated once every three days at a concentration of 100 mg/kg body weight in PBS. Mouse survival Kaplan Meier plot in %, body weight in grams, and tumor volume in ×10³ mm³ of each group are depicted in (a), (b), and (c), respectively; images of a representative mouse in each group were taken at d 40 and 70 (d). Bars, 1 cm.

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