Methanol and Butanol Extracts of Paeonia lutea Leaves Repress Metastasis of Squamous Cell Carcinoma

Figure 6

Effects of P. lutea leaf extracts on hepatic and pulmonary tumor-cell-metastasis in SAS-cell-xenograft nude mice. One million SAS cells were subcutaneously injected into the dorsal flank of nude mice, and vehicle (control), methanol (ME), ethyl acetate (EA), butanol (BU), butanol-insoluble (BW), and water (W) extracts of P. lutea leaves were administrated once every three days at 100 mg/kg body weight in PBS (see Section 2). After d 40 or 70, the mice were sacrificed, and the tumors, livers, and lungs were fixed and subjected to HE staining (HE) and immunohistochemistry for cytokeratin 10/13 (CK 10/13). Bars, 200 μm.