Endothelium-Dependent Vasorelaxant Effects of Dealcoholized Wine Powder of Wild Grape (Vitis coignetiae) in the Rat Thoracic Aorta

Figure 2

The effects of endothelium-derived relaxing factor inhibitors on WGWP-induced vasorelaxation in rat aortic rings. We pretreated endothelium-derived relaxing factors inhibitors, -nitro-L-arginine (NNA, nitric oxide synthase inhibitor, 10 μM), methylene blue (guanylate cyclase inhibitor, 1.0 M), and indomethacin (cyclooxygenase inhibitor, 10 μM), for 20 min and constricted rat aortic rings by 300 nM norepinephrine. Then, vasorelaxation was measured after treatment with WGWP 1.0 mg/mL. with different letters are significantly different () by one-way analysis of variance.