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Evidence-Based Complementary and Alternative Medicine
Volume 2016, Article ID 7043436, 12 pages
http://dx.doi.org/10.1155/2016/7043436
Research Article

Application of Partial Internal Transcribed Spacer Sequences for the Discrimination of Artemisia capillaris from Other Artemisia Species

1Division of Biological Sciences, Konkuk University, Seoul 143-701, Republic of Korea
2Department of Herbology, Wonkwang University, Iksan 570-749, Republic of Korea
3Korea Institute of Oriental Medicine, Daejeon 305-811, Republic of Korea

Received 11 December 2015; Revised 4 April 2016; Accepted 18 April 2016

Academic Editor: Yibin Feng

Copyright © 2016 Eui Jeong Doh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Several Artemisia species are used as herbal medicines including the dried aerial parts of Artemisia capillaris, which are used as Artemisiae Capillaris Herba (known as “Injinho” in Korean medicinal terminology and “Yin Chen Hao” in Chinese). In this study, we developed tools for distinguishing between A. capillaris and 11 other Artemisia species that grow and/or are cultured in China, Japan, and Korea. Based on partial nucleotide sequences in the internal transcribed spacer (ITS) that differ between the species, we designed primers to amplify a DNA marker for A. capillaris. In addition, to detect other Artemisia species that are contaminants of A. capillaris, we designed primers to amplify DNA markers of A. japonica, A. annua, A. apiacea, and A. anomala. Moreover, based on random amplified polymorphic DNA analysis, we confirmed that primers developed in a previous study could be used to identify Artemisia species that are sources of Artemisiae Argyi Folium and Artemisiae Iwayomogii Herba. By using these primers, we found that multiplex polymerase chain reaction (PCR) was a reliable tool to distinguish between A. capillaris and other Artemisia species and to identify other Artemisia species as contaminants of A. capillaris in a single PCR.