Application of Partial Internal Transcribed Spacer Sequences for the Discrimination of Artemisia capillaris from Other Artemisia Species

Figure 7

Multiplex polymerase chain reaction products by using two or three combined primer sets from mixed genomic DNA isolated from two or three Artemisia species at different content ratios. Primer set AC F4/ACJ R3 amplified a 189 bp DNA marker to detect A. capillaris; AJ F1/AC R3 amplified a 176 bp DNA marker to detect A. japonica; Fb/R7 amplified a 254 bp DNA marker to detect A. princeps; 2F1/2F3 amplified a 365 bp DNA marker to detect A. iwayomogi. Lane 1: A. capillaris; Lane 2: A. capillaris and A. japonica (9 : 1); Lane 3: A. capillaris and A. japonica (19 : 1); Lane 4: A. capillaris and A. princeps (9 : 1); Lane 5: A. capillaris and A. princeps (19 : 1); Lane 6: A. capillaris and A. iwayomogi (9 : 1); Lane 7: A. capillaris and A. iwayomogi (19 : 1); Lane 8: A. capillaris, A. japonica, and A. princes (8 : 1 : 1); Lane 9: A. capillaris, A. japonica, and A. princeps (18 : 1 : 1); Lane 10: A. capillaris, A. japonica, and A. iwayomogi (8 : 1 : 1); Lane 11: A. capillaris, A. japonica, and A. iwayomogi (18 : 1 : 1); M: 100 bp ladder, yellow underline: 96 bp internal standard amplified by AYF/AYR primer set.