Ex Vivo Stromal Cell-Derived Factor 1-Mediated Differentiation of Mouse Bone Marrow Mesenchymal Stem Cells into Hepatocytes Is Enhanced by Chinese Medicine Yiguanjian Drug-Containing Serum

Figure 3

Differentiated cell morphology from P2 BM-derived MSCs. BM-derived MSCs (P2) were cultured with 15% normal serum (magnification: ×10) and were spindle-shaped, spiral-shaped, or whirlpool-shaped (a). MSCs cultured with 20 ng/mL HGF + 15% normal serum for 14 days (magnification: ×20) became polygonal and circular, partially resembling hepatocyte-like cells (b). MSCs cultured 20 ng/mL HGF + 50 ng/mL SDF-1 + 15% normal serum for 7 days (magnification: ×20) were polygonal and circular, partially resembling hepatocyte-like cells (c). MSCs cultured with 20 ng/mL HGF + 50 ng/mL SDF-1 + 20% Yiguanjian drug-containing serum for 5 days (magnification: ×20) were polygonal and circular, partially resembling hepatocyte-like cells (d). MSCs cultured with 20% Yiguanjian drug-containing serum for 14 days (magnification: ×10) (e).

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