Ex Vivo Stromal Cell-Derived Factor 1-Mediated Differentiation of Mouse Bone Marrow Mesenchymal Stem Cells into Hepatocytes Is Enhanced by Chinese Medicine Yiguanjian Drug-Containing Serum

Figure 4

Albumin (ALB) expression in differentiated BM-derived MSCs (P2). MSCs were cultured in the presence of 20 ng/mL HGF + 15% normal serum (a), 20 ng/mL HGF + 50 ng/mL SDF-1 + 15% normal serum (b), 20 ng/mL HGF + 50 ng/mL SDF-1 + 20% Yiguanjian decoction (c), or 20% Yiguanjian drug-containing serum (d), and ALB was visualized by ICC at the indicated time points (magnification ×40: (a): (a3)-(a4) and (c): (a1)-(a2); magnification ×20: (a): (a1)-(a2), (b, c): (a3)-(a4), and (d)). ALB positive staining was observed at day 14 of culture in HGF + normal serum-treated BM-derived MSCs, and ALB staining density increased until day 28 (a). ALB staining was observed at day 7 of culture in HGF + SDF-1 + normal serum-treated BM-derived MSCs, and ALB staining density increased until day 28 (b). ALB staining was observed at day 7 of culture in HGF + SDF-1 + Yiguanjian decoction-treated BM-derived MSCs, and ALB staining density increased until day 28 (c). ALB positive staining cells in YGJ group could be observed at day 14 after induction, and increased gradually to day 21, and there was a reduction at day 28 (d).

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