Ex Vivo Stromal Cell-Derived Factor 1-Mediated Differentiation of Mouse Bone Marrow Mesenchymal Stem Cells into Hepatocytes Is Enhanced by Chinese Medicine Yiguanjian Drug-Containing Serum
Table 2
Comparison of ALB and CK-18 expression of induced BM-MSCs by western blot.
Group
7 days
14 days
21 days
28 days
HGF + normal serum
ALB
0.33 ± 0.099
0.435 ± 0.064
0.51 ± 0.198
2.515 ± 0.375
CK-18
0.113 ± 0.064
0.443 ± 0.28
0.477 ± 0.294
1.087 ± 0.482
HGF + SDF-1 + normal serum
ALB
3.004 ± 0.142
4.346 ± 0.049
7.714 ± 0.073
11.257 ± 3.425
CK-18
0.099 ± 0.007
0.23 ± 0.013
0.238 ± 0.001
0.562 ± 0.21
HGF + SDF-1 + YGJ
ALB
0.769 ± 0.04
2.286 ± 0.038
2.411 ± 0.002
2.826 ± 0.014
CK-18
0.251 ± 0.167
0.387 ± 0.138
2.281 ± 0.741
2.712 ± 0.537
YGJ
ALB
0.452 ± 0.04
1.145 ± 0.09#
2.143 ± 0.088
1.685 ± 0.357
CK-18
0.312 ± 0.041
0.963 ± 0.145#
1.283 ± 0.104#
1.119 ± 0.198
The data are shown as mean ± SD. , compared with other times in the same induction group. : 14 days versus 7 days and 21 daysversus 14 days.