The Fruit Hull of Gleditsia sinensis Enhances the Anti-Tumor Effect of cis-Diammine Dichloridoplatinum II (Cisplatin)

Figure 5

FGS, combined with CDDP, suppresses LLC-derived tumor in mice. Male mice (/group) received subcutaneous LLC injection and 7 days later were treated with sham (normal saline: NS), FGS, or CDDP (3 mg/kg) without or with FGS for indicated periods. The volume (a) and weight (b) of the tumor were measured every other day (, , and , compared with the NS-treated group; , compared with CDDP- or FGS-treated group). The tumor was surgically removed and analyzed by western blotting for p21 (c), phospho-p53 (d), and p27 (e). Each band was quantitated by a densitometer and relative expression of each protein was calculated over β-actin. Each column represents the mean ± SEM of three measurements ( and , compared with the NS-treated group; , compared with the CDDP-treated group).

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