Quantification of Phytochemicals from Commercial Spirulina Products and Their Antioxidant Activities

Table 5

Free amino acid contents (mg/100 g fresh wt.) in different Spirulina.

(a)

Number	Amino acids	RT (min)	Molecular weight	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17
1	Aspartate	1.48	133.10	1.37	1.37	2.03	2.66	5.24	1.34	3.66	1.24	2.47	0.97	3.55	4.05	3.33	0.89	1.06	1.69	1.70
2	Glutamate	2.77	147.13	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND
3	Asparagine	5.83	132.12	0.25	0.25	ND	0.92	0.39	ND	0.51	4.07	0.32	0.42	0.27	0.17	0.30	0.23	0.28	0.43	0.46
4	Serine	6.03	105.09	1.69	1.69	0.49	1.17	1.14	1.29	1.59	2.02	1.58	1.37	0.92	1.40	0.57	1.61	1.86	2.12	1.18
5	Vitamin U	6.59	199.70	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND
6	Glutamine	6.84	146.15	0.64	0.64	ND	1.89	3.25	0.55	1.46	0.66	ND	0.53	2.12	ND	1.09	ND	0.65	ND	0.98
7	Histidine	7.15	155.15	ND	ND	ND	0.72	ND	0.43	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND
8	Glycine	7.36	75.07	2.53	2.53	0.51	3.51	1.28	1.45	1.77	2.30	1.98	3.05	ND	1.59	1.02	2.04	2.36	2.71	2.28
9	Threonine	7.62	119.12	1.54	1.54	0.45	2.12	0.98	0.78	1.71	1.28	1.24	1.45	1.92	1.48	0.84	1.07	1.08	1.63	2.08
10	Arginine	8.47	174.20	2.81	2.81	1.87	1.87	2.82	4.00	3.04	2.20	5.12	4.70	1.55	2.81	1.59	2.31	2.48	5.12	3.02
11	Alanine	8.97	89.09	ND	ND	ND	11.68	ND	ND	ND	ND	ND	ND	ND	8.12	ND	ND	9.01	16.12	12.94
12	GABA	9.26	103.12	0.38	0.38	0.21	1.54	ND	0.32	0.35	1.26	1.00	0.84	ND	0.72	0.46	0.32	0.34	1.05	1.32
13	Tyrosine	10.43	181.19	2.08	2.08	0.46	3.04	0.69	1.70	1.81	1.43	ND	2.54	ND	1.66	ND	1.24	1.50	2.19	1.28
14	Cystine	12.26	240.30	ND	ND	ND	1.45	ND	ND	ND	ND	1.30	ND	0.45	ND	0.86	ND	ND	ND	0.52
15	Valine	12.56	117.15	2.13	2.13	0.72	5.34	0.76	1.92	2.51	ND	ND	1.07	ND	2.68	ND	1.42	1.20	4.02	3.19
16	Methionine	12.82	149.21	ND	ND	ND	0.80	ND	ND	ND	ND	ND	ND	0.93	ND	ND	ND	0.43	ND	ND
17	Norvaline	13.23	117.15	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND
18	Tryptophan	13.88	204.33	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND
19	Phenylalanine	14.37	165.19	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND
20	Isoleucine	14.60	131.17	1.98	1.98	0.44	4.31	0.69	1.69	2.23	1.13	2.01	0.62	0.39	2.07	0.61	0.89	0.77	2.79	2.00
21	Leucine	15.43	131.17	2.77	2.77	0.54	5.96	1.14	2.68	3.33	1.21	2.63	0.88	0.53	2.87	0.74	1.15	1.40	3.83	3.29
22	Lysine	16.05	146.19	1.68	1.68	0.38	4.04	0.79	0.00	2.44	1.63	1.90	2.21	0.71	1.70	0.72	1.38	1.45	2.95	1.63
Total				21.86	21.86	8.09	53.04	19.18	18.16	26.41	20.44	21.56	20.65	13.34	31.32	12.13	14.55	25.87	46.66	37.88

(b)

Number	Amino acids	RT (min)	Molecular weight	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37
1	Aspartate	1.48	133.10	2.76	3.21	1.44	2.88	1.45	0.71	2.75	0.46	1.10	1.87	1.98	1.56	0.56	1.21	1.16	1.62	1.28	0.49	1.94	1.02
2	Glutamate	2.77	147.13	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND
3	Asparagine	5.83	132.12	0.89	0.96	0.42	0.97	ND	ND	0.22	0.39	0.21	ND	ND	0.32	ND	0.35	ND	0.24	1.26	ND	0.52	0.37
4	Serine	6.03	105.09	1.12	1.91	0.92	1.04	2.17	1.11	0.56	0.69	1.14	1.12	1.96	1.01	0.62	1.02	1.01	1.11	3.17	0.79	1.74	1.15
5	Vitamin U	6.59	199.70	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND
6	Glutamine	6.84	146.15	1.31	1.52	0.98	1.87	ND	ND	1.07	ND	ND	ND	ND	0.53	ND	ND	0.57	ND	ND	ND	ND	ND
7	Histidine	7.15	155.15	0.48	0.56	ND	0.91	ND	ND	ND	ND	ND	ND	ND	ND	ND	0.30	ND	ND	0.53	ND	0.39	0.31
8	Glycine	7.36	75.07	2.65	3.13	1.96	3.95	1.75	1.69	1.08	2.04	2.27	2.19	3.37	1.41	1.46	2.24	2.31	1.51	6.33	1.47	5.37	2.03
9	Threonine	7.62	119.12	2.83	3.63	1.84	2.34	1.30	0.72	0.73	0.58	0.94	1.57	1.61	1.09	0.69	2.10	1.71	1.04	1.74	0.70	1.34	1.19
10	Arginine	8.47	174.20	2.52	3.12	1.98	2.35	5.37	2.47	1.74	1.64	2.19	2.66	3.58	2.66	1.34	2.80	3.01	2.41	8.46	1.88	2.63	2.91
11	Alanine	8.97	89.09	9.27	13.57	8.60	12.80	11.68	9.04	ND	8.03	9.26	9.84	12.89	4.36	7.60	15.30	11.57	6.51	23.85	6.03	16.34	6.24
12	GABA	9.26	103.12	1.26	1.83	0.99	1.35	0.48	1.79	0.20	1.74	1.13	1.63	4.36	0.44	6.72	1.16	0.57	0.44	0.77	0.23	2.35	0.97
13	Tyrosine	10.43	181.19	2.33	1.36	1.21	3.30	2.22	1.44	0.64	1.51	1.84	1.93	3.26	1.36	1.09	1.17	1.71	1.63	4.45	1.13	2.20	1.82
14	Cystine	12.26	240.30	1.30	0.80	0.65	1.64	0.38	ND	ND	ND	ND	ND	ND	ND	ND	0.76	ND	ND	0.57	ND	0.78	ND
15	Valine	12.56	117.15	4.34	5.38	2.43	6.05	2.97	1.49	0.70	1.29	1.69	2.65	3.05	1.19	1.62	3.60	3.42	1.67	16.12	ND	4.41	1.44
16	Methionine	12.82	149.21	0.69	0.35	ND	0.98	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND
17	Norvaline	13.23	117.15	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND
18	Tryptophan	13.88	204.33	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	0.43	ND	ND	0.64	ND	0.69	ND
19	Phenylalanine	14.37	165.19	2.76	2.53	1.13	3.36	1.42	0.83	ND	0.42	0.46	0.38	0.98	0.48	0.62	1.65	0.87	0.59	2.93	0.62	1.73	0.74
20	Isoleucine	14.60	131.17	2.95	3.43	1.29	3.98	2.07	0.95	0.44	0.92	1.05	1.50	1.70	0.85	1.06	1.92	2.67	1.11	ND	0.68	ND	ND
21	Leucine	15.43	131.17	4.74	5.82	2.41	6.59	3.99	1.81	0.71	1.03	1.21	1.47	1.95	1.24	1.61	3.32	3.35	1.62	7.59	0.96	3.14	1.23
22	Lysine	16.05	146.19	2.88	3.01	1.08	4.47	2.48	1.63	0.65	1.62	1.61	1.21	2.70	1.16	0.78	1.68	1.40	1.36	6.27	1.08	2.74	1.59
Total				47.05	56.14	29.35	60.83	39.74	25.68	11.49	22.34	26.10	30.01	43.38	19.65	25.77	41.00	35.33	22.85	85.97	16.08	48.31	23.01

Numbers 1 to 37 were the sample names (Table 1). ND: not detected.