Isolation of 4,5-O-Dicaffeoylquinic Acid as a Pigmentation Inhibitor Occurring in Artemisia capillaris Thunberg and Its Validation In Vivo

Figure 5

Inhibitory effect of the A. capillaris methanol extract, active fraction ACMF09, and 4,5-diCQA on pigmentation in developing zebrafish embryos. (a) Zebrafish was treated with test samples from 9 hfp to 72 hpf. Treatment with test samples at the indicated concentrations resulted in decreased pigmentation, as indicated by imaging the dorsal view of live embryos and the head portion. Scale bar = 250 µm. (b) Melanin content in zebrafish embryos treated with test samples from 9 hfp to 48 hpf. (c) Tyrosinase activity in the treated zebrafish. PTU was used as positive control. Results are expressed as percentages of the control, and the data are mean ± SEM of three independent experiments. compared to the untreated control.

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